| Construction and Identification of Plasmid pTA—TUB2 |
| |
| 作者姓名： | CHI Yu jie YANG Qian LI Ji chang |
| |
| 作者单位： | 1 Northeast Agricultural University，Harbin，Heilongjiang，150030，PRC; 2 Harbin Institute of Technology，Harbin，Heilongjiang，150001，PRC |
| |
| 基金项目： | Heilongjiang Province Natural Science fund( Grant No.C0 2 0 3 ) |
| |
| 摘 要： | 1 Introduction Benzamidazole fungicide plays an important role in plant disease control.However,as systemic fungi-cide,due to a long time application,it is facing a serious challenge from resistance problem.For instance,the resistance of Cercosporabeticola〔1〕 ,Penicillium digitatium Sacc.〔2〕Pyricularia oryzaecav〔3〕 to benzami-dazole fungicides was1 0 0 0 ︼g· m L-1.The resistance of wheat scab( Fusarium graminis)〔4〕,Botrytiscinerea〔5〕 and Sclerotinia sclerotiorum〔6…
|
| 关 键 词： | TUB2基因 PCR 基因转化 pRB129质粒 毛壳属 |
Construction and Identification of Plasmid pTA-TUB2 |
| |
| CHI Yu jie ,YANG Qian ,LI Ji chang .Construction and Identification of Plasmid pTA-TUB2[J].Journal of Northeast Agricultural University,2003,10(1):78-83. |
| |
| Authors: | Chi Yu-jie YANG Qian LI Ji-chang |
| |
| Abstract: | An about 1.40 Kb target gene fragment was yielded by PCR amplification with the plasmid pRB 129,which was identified by restriction enzyme digestion that the PCR product was TU B2 gene.The gene was digested by the restriction enzyme and was linked with pTA plasmid to construct pTA-TU B2 plasmid.The plasmid was transformed into Chaetomium spp.by PEG method and the transformation rate was 27/(2×105) and it is nine times higher than that of pRB 129.The transformants can grow on the PDA containing 1 000 μg*mL-1 carbendazim,which is 1 000 times higher than the original Chaetomium spp.The resistance was stable after 10 times transfer on non-selective medium. |
| |
| Keywords: | resistance gene to carbendazim plasmid construction Chaetomium spp transformation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
