| 反相高效液相色谱法测定辐花肋柱花中苦龙苷含量的研究 |
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| 引用本文： | 俞青芬,林鹏程.反相高效液相色谱法测定辐花肋柱花中苦龙苷含量的研究[J].安徽农业科学,2007,35(4):967-968. |
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| 作者姓名： | 俞青芬 林鹏程 |
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| 作者单位： | 青海民族学院化学系,青海西宁,810007;青海民族学院药学系,青海西宁,810007 |
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| 摘 要： | 建立了反相高效液相色谱法测定辐花肋柱花中苦龙苷的含量.采用Kromasil-C18(2 50 mm×4.6 mm,5 μm)的色谱柱,以甲醇-水(52∶48,体积比)为流动相等度洗脱,流速0 .8 ml/min,检测波长254 nm,柱温30 ℃.结果表明,在试验条件下苦龙苷与其他成分达到基线分离.苦龙苷的线性范围为0.028～0.165 μg(r=0.9995).该方法简便、快速,结果准确、可靠.
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| 关 键 词： | 反相高效液相色谱 苦龙苷 辐花肋柱花 龙胆科 |
| 文章编号： | 0517-6611（2007）04-00967-02 |
| 修稿时间： | 2006-10-31 |
Determination of Amarogerth in Lomatogonium Rotatum by RP-HPLC |
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| YU Qing-fen et al.Determination of Amarogerth in Lomatogonium Rotatum by RP-HPLC[J].Journal of Anhui Agricultural Sciences,2007,35(4):967-968. |
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| Authors: | YU Qing-fen |
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| Institution: | Chemistry Deparment, Qinghai Institute of Nationalities, Xining, Qinghai 810007 |
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| Abstract: | In this article,RP-HPLC was applied to determine the content of amarogerth in Lomatogonium rotatum.The sample were separated on the column of Kromasil-C18(250 mm×4.6 mm,5 μm) which eluted with methanol and water(0.04% phosphoric acid).The ratio of methanol and water was 52∶48 with detected wavelength at 254 nm,flow rate at 0.8 ml/min,column temperature at 30 ℃.The result showed that the compound was mostly separated.The linear ranges of amarogerth was 0.028～0.165 μg(r=0.999 5).This method was rapid and accurate. |
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| Keywords: | RP-HPLC Amarogerth Lomatogonium rotatum Gentianaceae |
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