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| 鲢鱼嗜水气单胞菌PCR检测方法的建立 | |
| 引用本文： | 余晓丽,秦春香,陈明,熊艳芸,雷爱莹,黄婷,李超,王瑞,梁万文.鲢鱼嗜水气单胞菌PCR检测方法的建立[J].广西农业科学,2008,39(5). |
| 作者姓名： | 余晓丽 秦春香 陈明 熊艳芸 雷爱莹 黄婷 李超 王瑞 梁万文 |
| 作者单位： | 1. 广西水产研究所,南宁,530021 2. 广西水产畜牧学校,南宁,530021 3. 广西水产研究所,南宁,530021;广西大学动物科学技术学院,南宁,530005 |
| 摘 要： | 根据基因库中嗜水气单胞菌16SrRNA基因序列,设计并合成一对特异性引物,用PCR方法对从病死鲢鱼体内分离到的1株嗜水气单胞菌进行扩增,并对PCR反应条件进行优化,同时测试了该方法的特异性和敏感性.特异性试验结果显示,该引物能扩增出680bp的嗜水气单胞菌特异基因片段,与鳗弧菌、溶藻弧菌、副溶血弧菌、温和气单胞菌、迟缓爱德华氏菌、HT5",7"]鱼KG-*3]回HT5"]爱德华氏菌及海豚链球菌无交叉反应;敏感性试验结果显示,该方法最低检测量为10pg嗜水气单胞菌基因总DNA.表明该实验所建立的PCR检测方法可用于鲢鱼嗜水气单胞菌的快速诊断,对有效治疗和控制鱼类嗜水气单胞菌病的流行具有重要意义. |
| 关 键 词： | 鲢鱼 嗜水气单胞菌 PCR检测 特异性 敏感性 |
Development of a diagnostic PCR assay for Aeromonas hydrophila of hypophthalmichthys molitrix |
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| YU Xiao-li,QIN Chun-xiang,CHEN Ming,XIONG Yan-yun,LEI Ai-ying,HUANG Ting,LI Chao,WANG Rui,LIANG Wan-wen.Development of a diagnostic PCR assay for Aeromonas hydrophila of hypophthalmichthys molitrix[J].Guangxi Agricultural Sciences,2008,39(5). | |
| Authors: | YU Xiao-li QIN Chun-xiang CHEN Ming XIONG Yan-yun LEI Ai-ying HUANG Ting LI Chao WANG Rui LIANG Wan-wen |
| Institution: | YU Xiao-li1,QIN Chun-xiang1,CHEN Ming1,XIONG Yan-yun2,LEI Ai-ying1,HUANG Ting1,LI Chao1,3,WANG Rui1,LIANG Wan-wen1*(1 Guangxi Fisheries Research Institute,Nanning 530021,China,2 Guangxi Aquatic , AnimalHusb,ry School,3 College of Animal Science , Technology,GuangxiUniversity,Nanning 530005,China) |
| Abstract: | A pair of specific primers was designed and synthesized according to 16S RNA gene sequences of Aeromonas hydrophila from GenBank.The specific DNA fragment of an Aeromonas hydrophila strain separated from dead disease hypophthalmichthys molitrix was amplified by using PCR.The PCR reaction condition was optimized and the specificity and sensitivity of this PCR method were tested.The results of specificity test indicated that this primer could amplify 680 bp specific DNA fragment from Aeromonas hydrophila and ... |
| Keywords: | hypophthalmichthys molitrix Aeromonas hydrophila PCR detection |
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