| 幼丹顶鹤感染血孢子虫的PCR鉴定 |
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| 引用本文： | 贾婷,张成林,刘贤勇,杨明海,郝力力,罗毅,阎文朝,普天春,石团员,夏茂华,吴秀山,索勋,张金国.幼丹顶鹤感染血孢子虫的PCR鉴定[J].野生动物,2010,31(6):323-326,337. |
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| 作者姓名： | 贾婷 张成林 刘贤勇 杨明海 郝力力 罗毅 阎文朝 普天春 石团员 夏茂华 吴秀山 索勋 张金国 |
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| 作者单位： | [1]北京动物园,北京100044 [2]中国农业大学动物医学院,北京100193 |
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| 摘 要： | 2006～2008年,北京动物园繁殖的17只1.5～3.5月龄丹顶鹤幼鹤出现厌食、精神倦怠、呼吸困难等症状。采用PCR方法鉴定病原,使用特异性引物对线粒体细胞色素b、细胞色素氧化酶Ⅰ、18S rRNA及线粒体部分非编码区基因进行扩增、克隆、测序,结合GenBank中已知疟原虫序列,经DNAMAN软件处理分析,并对Cytb序列用软件MEGA 4.0建NJ树。序列分析表明丹顶鹤血孢子虫靶序列与鸟疟原虫序列相似性达到98%以上,进化关系分析表明与残疟原虫各株属同一个种群,证明该病病原为残疟原虫。但该血孢子虫与残疟原虫已报道各株形态有一定差异,可能为寄生于丹顶鹤的一个新基因型。
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| 关 键 词： | 丹顶鹤 血孢子虫 PCR 残疟原虫 |
PCR Determination of Haemosporidian Parasites, infecting Juvenile Red-crowned Crane in Beijing Zoo |
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| Authors: | Jia Ting Zhang Chenglin Liu Xianyong Yang Minghai Hao Lili Luo Yi Yan Wenchao Pu Tianchun Shi Tuanyuan Xia Maohua Wu Xiushan Suo Xun Zhang Jinguo |
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| Institution: | 1＊) (1.Beijing Zoo,Beijing,100044,China;2.Parasitology Laboratory,College of Veterinary Medicine,China Agricultural University,Beijing,100193,China) |
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| Abstract: | Diseased juvenile red - crowned cranes（Grus japonensis） in Beijing Zoo was diagnosed by PCR amplification of mitochondrial genes the parasite：cytochrome b gene（Cyt6）,Cytochrome oxidase 1（CO 1）,18S rRNA gene and the amplified DNA fragments were cloned and sequenced and constructed a phylogenetic tree with the published DNA sequences of Plasmodium sp.,Leucocytozoon sp.and Haemoproteus sp.in GenBank,by software MEGA 4.0.DNA sequence comparison indicated that the highest sequence homology,98%was between the cloned Cyt6 gene and that of Plasmodium retictum so the parasite was determined to be a member of this species,though there were morphological differences between infected parasite and the reported Plasmodium relictum in the world. |
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| Keywords: | Red-crowned crane Haemosporidian PCR technique Plasmodium relictum |
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