| 东毕吸虫成虫抗原纯化的试验研究 |
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| 引用本文： | 耿进明,李泽鸿.东毕吸虫成虫抗原纯化的试验研究[J].畜牧兽医学报,1997,28(4):357-361. |
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| 作者姓名： | 耿进明 李泽鸿 |
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| 作者单位： | 吉林农业大学动物科学系 |
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| 摘 要： | 本研究首次以葡萄聚糖Ｇ２００纯化东毕吸虫成虫原，用７５１分光光度计测各管的ＯＤ值，结果共出现四个洗脱峰，其中以第一峰峰值最高（ＯＤ２８０＝１．７５），蛋白浓度为１．３２９ｍｇ／ｍｌ。将纯化的抗原与东毕吸虫阳性血清，阴性血清采用ＩＨＡ的方法测定其抗原的特异性与敏感性。
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| 关 键 词： | 东毕吸虫病 抗原纯化 牛病 |
EXPERIMENTAL STUDY ON THE ANTIGEN PURIFICATION FROM ADULT ORIENTOBILHARIZIA SPP |
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| Geng Jinming,Li Zehong,et al.EXPERIMENTAL STUDY ON THE ANTIGEN PURIFICATION FROM ADULT ORIENTOBILHARIZIA SPP[J].Acta Veterinaria et Zootechnica Sinica,1997,28(4):357-361. |
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| Authors: | Geng Jinming Li Zehong et al |
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| Abstract: | The adult antigen from orientobilharizia spp,was first purified by using sephadex G 200 in this study The OD value of each test tube was determined by using 751 spectrophotometer,and 4 different peaks were obtained The highest OD value (OD=1 75) occurred in the first peak and the protein concentration was 1 329mg/ml Using IHA,the speciality and sensitivity of the purified antigen were determined according to the reaction between the antigen and positive,negative sera to orentobilharizia spp The results showed that,the purified antigen(the first peak) had the characteristics of high speciality (93 3%) and sensitivity (agglutination reaction still occurred between the antigen and antibody after 1:1024 dilution of positive sera |
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| Keywords: | Orientobilhariziosis in cattle Antigen purification |
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