| 香蕉MuMADS2基因表达产物的亚细胞定位 |
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| 引用本文： | 刘琳,杨晓颖,金志强,刘菊华,张浩,徐碧玉.香蕉MuMADS2基因表达产物的亚细胞定位[J].热带作物学报,2010,31(5):777-781. |
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| 作者姓名： | 刘琳 杨晓颖 金志强 刘菊华 张浩 徐碧玉 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101;海南大学农学院,海南,海口,571101
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101 |
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| 基金项目： | 国家自然科学基金，香蕉公益性行业科研专项，中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 对已获得的香蕉MuMADS2基因进行生物信息学和芯片分析表明,该基因编码的蛋白可能作为转录因子定位于细胞核中,是乙烯上游的调控因子,参与调控香蕉果实的成熟过程。为进一步研究该基因功能,构建香蕉MuMADS2基因与绿色荧光蛋白(Green Fluorescent Protein,GFP)基因融合的植物表达载体pCAMBIA1302-MuMADS2,再利用基因枪转化法将其转入洋葱表皮细胞进行亚细胞定位观察。结果表明:该基因表达产物定位于细胞核中,符合转录因子特性。
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| 关 键 词： | 香蕉 MuMADS2基因 转化 亚细胞定位 |
Subcellular Localization of Expression Products of Banana MuMADS2 Gene |
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| Liu Lin,Yang Xiaoying,Jin Zhiqiang,Liu Juhu,Zhang Hao and Xu Biyu.Subcellular Localization of Expression Products of Banana MuMADS2 Gene[J].Chinese Journal of Tropical Crops,2010,31(5):777-781. |
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| Authors: | Liu Lin Yang Xiaoying Jin Zhiqiang Liu Juhu Zhang Hao and Xu Biyu |
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| Institution: | 1 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science 2 Department of Agriculture, Hainan University;1 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science 2 Department of Agriculture, Hainan University;Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science;Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science;1 Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science 2 Department of Agriculture, Hainan University;Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agricultural Science |
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