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响应面法优化鱼鳞脱钙工艺的研究
引用本文:黄煜,王茵,阮伟达,吴成业.响应面法优化鱼鳞脱钙工艺的研究[J].福建水产,2012,34(2):127-133.
作者姓名:黄煜  王茵  阮伟达  吴成业
作者单位:1. 福建农林大学食品科学学院,福建福州350002/福建省水产研究所,福建厦门361013
2. 福建省水产研究所,福建厦门,361013
3. 福建省水产研究所,福建厦门361013/集美大学生物工程学院,福建厦门361021
基金项目:福建省水产研究所科研发展基金资助项目
摘    要:利用响应面分析法对罗非鱼(Tilapia mossambica)鱼鳞的脱钙工艺进行优化。以鱼鳞脱钙率为指标,选取盐酸浓度、底物浓度、反应温度及反应时间为影响因子,进行单因素试验,筛选出适当的因素和水平,根据Box-Behnken中心组合设计,按照所建立的动态方程模型,分析各因素的显著性和交互作用,得出罗非鱼鱼鳞脱钙的最佳工艺条件为:盐酸浓度2.9%,底物浓度6.3%,反应时间1.9 h,该条件下鱼鳞脱钙率为99.55%。

关 键 词:鱼鳞  脱钙  响应面分析  优化

Optimization of decalcification technique of fish scale by response surface methodology
HUANG Yu,WANG Yin,RUAN Wei-da,WU Cheng-ye.Optimization of decalcification technique of fish scale by response surface methodology[J].Journal of Fujian Fisheries,2012,34(2):127-133.
Authors:HUANG Yu  WANG Yin  RUAN Wei-da  WU Cheng-ye
Institution:1.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China; 2.Fisheries Research Institute of Fujian,Xiamen 361013,China; 3.Jimei University,Xiamen 361022,China)
Abstract:Response surface analysis was applied to optimize the decalcification technique of Tilapia mossambica fish scale.Hydrochloric acid concentration,substrate concentration,reaction temperature and reaction time were chosen as the impact factors based on decalcification rate of fish scale,and the appropriate levels and factors were selected in previous single factor experiments.According to the Box-Behnken central composite design,in accordance with the dynamic equation model,the significance of all factors and interactions among them were analyzed.The optimum condition of decalcification technique of Tilapia mossambica fish scale was obtained as follows: hydrochloric acid concentration of 2.9%,substrate concentration of 6.3%,reaction time of 1.9 h,and the decalcification rate of fish scale could reach 99.55% under this condition.
Keywords:fish scale  decalcification  response surface analysis  optimization
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