| 栗属植物cpSSR标记技术的建立与体系优化 |
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| 引用本文： | 甄贞,曹庆芹,杨凯,沈元月,冯永庆,秦岭.栗属植物cpSSR标记技术的建立与体系优化[J].果树学报,2007,24(4):557-560. |
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| 作者姓名： | 甄贞 曹庆芹 杨凯 沈元月 冯永庆 秦岭 |
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| 作者单位： | 1. 北京农学院植物科学技术系,北京,102206
2. 北京农学院生物技术系,北京,102206 |
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| 基金项目： | 北京市优秀人才培养基金
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北京农学院校科研和教改项目
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北京市属市管高校人才强教计划 |
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| 摘 要： | 叶绿体微卫星标记是一种新的分子标记技术。用1对来源于拟南芥的cpSSR引物研究栗属资源,对栗属植物cpSSR反应体系的主要影响因子进行了优化筛选。结果表明,20μL反应体系各组分的最适浓度分别为:2.5mmol/LMg2+,0.5U Taq DNA聚合酶,0.25mmol/L dNTP,10mg/L的DNA模板,正向引物和反向引物都为0.1μmol/L,NTCP40最适退火温度为56℃。该体系已成功应用于栗属资源的遗传多样性研究中。
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| 关 键 词： | 栗属植物 cpSSR 体系优化 |
| 文章编号： | 1009-9980（2007）04-557-04 |
| 修稿时间： | 2006-12-30 |
Establishment of cpSSR marker technology and optimization of its reaction system in genus Castanea |
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| ZHEN Zhen,CAO Qing-qin,YANG Kai,SHEN Yuan-yue,FENG Yong-qing,QIN Ling.Establishment of cpSSR marker technology and optimization of its reaction system in genus Castanea[J].Journal of Fruit Science,2007,24(4):557-560. |
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| Authors: | ZHEN Zhen CAO Qing-qin YANG Kai SHEN Yuan-yue FENG Yong-qing QIN Ling |
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| Institution: | 1.Department of Plant Science and Technology; 2.Department of Biotechnology ,Beijing Agricultural College, Beijing 102206 China |
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| Abstract: | Chloroplast microsatellite is a new molecular marker technology.Experiment was conducted with cpSSR primers obtained from Arabidopsis thaliana for studying the germlasm of genus Castanea Mill.The optimization of main influential factors of cpSSR reaction system in Castanea was studied.The results showed that the optimum concentrations of Mg2+,Taq DNA polymerase,dNTPs,template DNA,and primers in 20 μL reaction system were 2.5 mmol/L,0.5 U,0.25 mmol/L,10 mg/L and 0.1 μmol/L,respectively.56 ℃ was the most suitable annealing temperature of NTCP40 primer.Moreover,it was successful in studying genetic diversity of germplasm of Castanea Mill. |
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| Keywords: | Castanea Mill cpSSR Optimization of reaction system |
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