Induction of mutations for heat tolerance in potato by using in vitro culture and radiation |
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| Authors: | A Das SS Gosal JS Sidhu HS Dhaliwal |
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| Institution: | (1) Department of Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India |
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| Abstract: | Heat tolerant mutants were obtained in two commercial potato cultivars, `Kufri Jyoti' and `Kufri Chandramukhi' through in vitro mutagenesis of in vitro propagated plantlets. Gamma-irradiated (20 and 40 Gy) shoots were micropropagated for three cycles (M1V3). A large number of the micropropagated shoots produced microtubers at 28 °C. Microtubers induced at high temperature had distorted shape but showed normal germination in field. Under stress conditions
of high temperature, the frequency of chlorophyll variants increased in the gamma irradiation-derived material, however, nearly
40% of the plants had normal leaf tissue, whereas control plants showed completely damaged leaves.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | in vitro culture gamma rays heat tolerance potato |
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