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苯丙烷代谢与嫁接黄瓜抗南方根结线虫的关系
引用本文:张宇,刘世琦,张自坤,杨晓建,马琳,黄治军,陈昆.苯丙烷代谢与嫁接黄瓜抗南方根结线虫的关系[J].中国蔬菜,2010,1(22):54-58.
作者姓名:张宇  刘世琦  张自坤  杨晓建  马琳  黄治军  陈昆
作者单位:山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
基金项目:公益性行业(农业)科研专项经费项目,国家科技支持计划项目
摘    要:采用盆栽幼苗人工接种的方法,对感染南方根结线虫的黄瓜嫁接苗和砧木的苯丙烷代谢产物及关键酶等指标进行了测定,以研究苯丙烷代谢与嫁接黄瓜抗根结线虫的关系。试验结果表明:接种南方根结线虫后,黄瓜嫁接苗木质素和总酚含量显著增加,PAL、TAL、PPO活性显著提高,纤维素含量变化不大,根结线虫指数明显下降,表明苯丙烷代谢的加速与黄瓜抗南方根结线虫密切相关;不同砧木具有不同的抗南方根结线虫的能力,6种砧木抗南方根结线虫能力依次为:DTT-RS-39(T4)改良火凤凰(T0)火凤凰(T1)崛京隆(T3)优清台木(T2)黑籽南瓜(T5)。

关 键 词:南方根结线虫  嫁接  苯丙烷代谢  黄瓜砧木  
收稿时间:2010-08-24;

Relationship between Phenylpropanes Metabolism and Grafted Cucumber Resistance to Southern Root-knot Nematode
ZHANG Yu,LIU Shi-qi,ZHANG Zi-kun,YANG Xiao-jian,MA Lin,HUANG Zhi-jun,CHEN Kun.Relationship between Phenylpropanes Metabolism and Grafted Cucumber Resistance to Southern Root-knot Nematode[J].China Vegetables,2010,1(22):54-58.
Authors:ZHANG Yu  LIU Shi-qi  ZHANG Zi-kun  YANG Xiao-jian  MA Lin  HUANG Zhi-jun  CHEN Kun
Institution:College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, China
Abstract:To find out the relationship between the resistance to root-knot nematode and phenylpropanes metabolism in grafted cucumber(Cucumis sativus L.)seedlings, the effects of infection of southern root-knot nematode on phenylpropanes and key enzymes index were measured by artificial grafting of pot cucumber seedlings. The results showed that after infected by southern root-knot nematode, the contents of total phenols and lignin, and the activities of phenylalanine ammonia-lyase(PAL), tyrosine ammonia lyase(TAL)and polyphenol oxidase(PPO)were significantly higher than those in self-root seedlings, while the contents of cellulose had little change and the index of root-knot nematode even declined. We concluded that phenylpropane metabolites and key enzymes had close correlation with resistance to southern root-knot nematode in grafted cucumber seedlings. Further studies disclosed that the resistances of 6 rootstocks to southern root-knot nematode were different with the sequence of DTT-RS-39(T4)>‘Gailianghuofenghuang’(T0)>‘Huofenghuang’(T1)>‘Juejinglong’(T3)>‘Youqingtaimu’(T2)>‘Heizinangua’(T5).
Keywords:Graft  Phenylpropane metabolism  Cucumber rootstocks
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