| 源于奶牛蹄部感染的牛源污蝇解壳杆菌的分离鉴定与药敏试验 |
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| 引用本文： | 王志浩,郭龙,刘康军,李俊,李建基,崔璐莹,孟霞,朱国强,钱晨,王亨.源于奶牛蹄部感染的牛源污蝇解壳杆菌的分离鉴定与药敏试验[J].中国兽医学报,2021(1):86-90. |
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| 作者姓名： | 王志浩 郭龙 刘康军 李俊 李建基 崔璐莹 孟霞 朱国强 钱晨 王亨 |
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| 作者单位： | ;1.扬州大学兽医学院/江苏省动物重要疫病与人兽共患病防控协同创新中心 |
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| 基金项目： | 江苏现代农业产业技术体系建设专项资金资助项目(JATS[2019]461);科技部畜禽重大疫病防控与高效安全养殖综合技术研发重点专项资助项目(2016YFD0500905);江苏高校优势学科建设工程资助项目(PAPD);江苏高校品牌专业建设工程资助项目(TAPP)。 |
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| 摘 要： | 为确定导致江苏某奶牛场蹄部感染的病原,采集发病奶牛蹄部感染病灶的病料进行细菌分离培养,使用MALDI Biotyper微生物快速鉴定系统进行细菌鉴定,并对分离菌株进行药物敏感性试验。结果显示,病灶中的分离株为污蝇解壳杆菌(Wohlfahrtiimonas chitiniclastica),血平板上呈β型溶血。药敏试验结果显示,该菌株对阿莫西林钠-克拉维酸钾、多西环素、四环素、头孢氨苄、链霉素、青霉素和复方新诺明7种抗生素耐药,对多黏菌素、丁胺卡那、庆大霉素、环丙沙星、克林霉素5种抗生素敏感。污蝇解壳杆菌作为一种人畜共患的病原菌,其在奶牛蹄部的感染需要引起奶牛场的关注,同时其耐药性增加更具有公共卫生学意义。该病原的分离鉴定和药敏试验为指导奶牛养殖场合理用药和提高公共卫生管理具有重要意义。
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| 关 键 词： | 奶牛 污蝇解壳杆菌 分离鉴定 药敏试验 |
Isolation,identification and drug susceptibility testing of bovine Wohlfahrtiimonas chitiniclastica from infectious hooves of dairy cows |
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| WANG Zhihao,GUO Long,LIU Kangjun,LI Jun,LI Jianji,CUI Luying,MENG Xia,ZHU Guoqiang,QIAN Chen,WANG Heng.Isolation,identification and drug susceptibility testing of bovine Wohlfahrtiimonas chitiniclastica from infectious hooves of dairy cows[J].Chinese Journal of Veterinary Science,2021(1):86-90. |
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| Authors: | WANG Zhihao GUO Long LIU Kangjun LI Jun LI Jianji CUI Luying MENG Xia ZHU Guoqiang QIAN Chen WANG Heng |
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| Institution: | (Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University fYangzhou,Jiangsu 225009,China) |
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| Abstract: | To determine the cause of the infectious hooves in a dairy farm in Jiangsu,the samples from the affected hooves were collected for bacterial culture and isolation.The MALDI Biotyper rapid identification system was used for bacterial identification and the drug sensitivity test was performed on the isolated strain.The results showed that the isolated strain was Wohlfahrtiimonas chitiniclastica,andβ-type hemolysis was found on the blood plate.Drug susceptibility test results showed that the isolated strain was resistant to seven antibiotics including amoxicillin sodium-clavulanate potassium,doxycycline,tetracycline,cephalexin,streptomycin,penicillin,and compound sinomine,and sensitive to five antibiotics including clindamycin,polymyxin,tetracycline,gentamicin,and ciprofloxacin.Wohlfahrtiimonas chitiniclastica is a zoonotic pathogen,which can cause infections in the hooves of dairy cows.Therefore,this problem should be paid attention to in dairy farms.At the same time,the increase in drug resistance also has more public health significance.The isolation and identification of the pathogen and the drug sensitivity test are significant for guiding the rational use of drugs in dairy farms and improving public health management. |
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| Keywords: | dairy cow Wohlfahrtiimonas chitiniclastica isolation and identification drug sensitivity test |
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