| H9N2亚型流感病毒光动力失活的超微结构 |
| |
| 引用本文： | 谷长维,胡博.H9N2亚型流感病毒光动力失活的超微结构[J].中国兽医学报,2021(2):268-272. |
| |
| 作者姓名： | 谷长维 胡博 |
| |
| 作者单位： | ;1.中国农业科学院特产研究所;2.农业农村部经济动物疫病重点实验室 |
| |
| 基金项目： | 国家重点研发计划资助项目(2017YFD0501600)。 |
| |
| 摘 要： | 使用H9N2亚型流感病毒作为系统模型,用不同浓度(2,4μmol/L)的光敏剂经激光照射(12 J/cm2,20 min),使病毒颗粒失活后,使用透射电子显微镜(TEM)观察病毒粒子形态,并对病毒的感染性进行测定。结果显示,低浓度光敏剂处理组的病毒粒子膜结构虽然保持完整性,但表面糖蛋白缺失,同时丧失了对MDCK细胞的感染性;高浓度光敏剂处理组的病毒粒子膜结构不完整,出现不同程度的膜结构损伤,病毒粒子对MDCK细胞无感染性。本试验为流感病毒的光动力失活提供了可靠的试验依据。
|
| 关 键 词： | 流感病毒 H9N2 光动力失活 光敏剂 透射电子显微镜 |
Ultrastructure characteristics of photodynamic inactivation of H9N2 influenza virus |
| |
| GU Changwei,HU Bo.Ultrastructure characteristics of photodynamic inactivation of H9N2 influenza virus[J].Chinese Journal of Veterinary Science,2021(2):268-272. |
| |
| Authors: | GU Changwei HU Bo |
| |
| Institution: | (Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Key Laboratory of Special Animal Epidemic Disease,Ministry of Agriculture and Affair Rural,Changchun 130112,China) |
| |
| Abstract: | H9 N2 influenza virus was used as a model system,the virus particles were treated with different concentrations(2,4μmol/L)of photosensitizer and irradiated by laser(12 J/cm2,20 min).After inactivating the virus particles,transmission electron microscopy was used to observe the morphology of virus particles,and the infectivity of the virus was determined.The results showed that although the membrane structure of virus particles treated with low concentration of photosensitizer remained intact,the surface glycoprotein of virus particles was absent,at the same time,H9 N2 influenza virus particles lost the infectivity to MDCK cells.The membrane structure of virus particles in high concentration photosensitizer treatment group was incomplete,and membrane structure was damaged in different degrees,and all virus particles had no infectivity to MDCK cells.This study provides a reliable experimental basis for photodynamic inactivation of influenza virus. |
| |
| Keywords: | influenza virus H9N2 photodynamic inactivation photosensitizer transmission electron microscopy |
| 本文献已被 维普 等数据库收录！ |
|
