| 西藏那曲牦牛源产气荚膜梭菌的分离鉴定及生物学特性 |
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| 引用本文： | 罗润波,贡嘎,高家登,扎西次仁,白玛桑珠,索朗斯珠.西藏那曲牦牛源产气荚膜梭菌的分离鉴定及生物学特性[J].中国兽医学报,2021(1):74-80. |
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| 作者姓名： | 罗润波 贡嘎 高家登 扎西次仁 白玛桑珠 索朗斯珠 |
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| 作者单位： | ;1.西藏农牧学院动物科学学院;2.西藏那曲市畜牧兽医技术推广总站;3.西藏那曲市班戈县农牧科学技术服务站 |
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| 基金项目： | 国家肉牛牦牛产业技术体系资助项目(CARS-37);西藏科技厅2019年度重点资助项目;西藏班戈县畜牧业综合发展关键技术研究基金资助项目;元亨基金资助项目。 |
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| 摘 要： | 利用细菌培养、生化鉴定、小鼠致病性试验、PCR技术等方法对西藏那曲市多起牦牛猝死病例进行诊断和分析。结果表明,从病死牦牛脏器中分离得到3株产气荚膜梭菌,命名为AD-01、AD-02和BG-01。其中AD-01和AD-02分离株属于A型,BG-01分离株属于C型。通过对分离株16S rRNA进行序列比对并制作进化树分析发现,AD-01和AD-02分离株为A型产气荚膜梭菌,与中国近年来报道的分离菌株遗传距离较远,属于完全独立的一个分支;同时药敏试验结果显示AD-01、AD-02分离株与BG-01分离株的药敏试验结果有一定差异,产生此现象的原因需进一步研究。本试验成功分离到3株牦牛源产气荚膜梭菌,为牦牛产气荚膜梭菌病的预防和控制提供了理论依据。
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| 关 键 词： | 牦牛 产气荚膜梭菌 分离鉴定 进化分析 药敏试验 |
Isolation,identification and biological characterization of Clostridium perfringens isolated from yak in Naqu,Tibet |
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| Institution: | (Department of Animal Science,Tibet Agricultural and Animal Husbandry College,Nyingchi,Tibet 860000,China;Animal Husbandry and Veterinary Technology Promotion Station of Naqu District,Naqu,Tibet 852000,China;Agriculture and Animal Husbandry Science and Technology Service Station of Bange County,Bange,Tibet 852500,China) |
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| Abstract: | We diagnosed and analyzed many cases of sudden death of yak in Naqu district,using bacterial culture,biochemical identification,mouse pathogenicity tests and PCR methods.The results showed that three strains of Clostridium perfringens were isolated in dead or sick yaks,named AD-01,AD-02,BG-01,respectively.The AD-01 strain and AD-02 strain were identified as type A.C.perfringens,while BG-01 was identified as type C.Furthermore,phylogenetic analysis of 16 S rRNA showed that AD-01,AD-02 strain clustered in an independent branch from China isolated strains.Antibiotic susceptibility assay indicated diversity between the AD-01,AD-02 and BG-01,the reason needed to be further investigated.Three strains of Clostridium perfringens from yaks were successfully isolated in this experiment,which provide a theoretical basis for the prevention and control of Clostridium perfringens in yaks. |
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| Keywords: | yak Clostridium perfringens isolation and indentification phylogenetic analysis antibiotic susceptibility assay |
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