| BTV-16四结构蛋白的共表达与病毒样颗粒的制备 |
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| 引用本文： | 黄超华,花群义,曹琛福,史卫军,阮周曦,林彦星,叶奕优,陶虹,王潇,陈金顶.BTV-16四结构蛋白的共表达与病毒样颗粒的制备[J].中国兽医学报,2021(1):1-7. |
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| 作者姓名： | 黄超华 花群义 曹琛福 史卫军 阮周曦 林彦星 叶奕优 陶虹 王潇 陈金顶 |
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| 作者单位： | 1.华南农业大学兽医学院;2.深圳海关动植物检验检疫技术中心 |
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| 基金项目： | 国家“十三五”重点研发计划资助项目(2017YFD0502304,2017YFD0501104)。 |
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| 摘 要： | 目前疫苗免疫是预防蓝舌病的主要有效手段,蓝舌病病毒样颗粒(VLPs)疫苗是蓝舌病疫苗研究的热点之一.为了制备蓝舌病病毒血清16型(BTV-16)病毒样颗粒,研究对载体pFastBac-Dual进行了改造,使其具有4个独立启动子的多克隆位点.依次将BTV-16 VP2、VP5、VP3和VP7基因插入到改造后载体(pF4)...
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| 关 键 词： | 蓝舌病 昆虫杆状病毒 BTV-16 病毒样颗粒 |
Co-expression of BTV-16 four structural proteins and preparation of virus-like particles |
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| HUANG Chaohua,HUA Qunyi,CAO Chenfu,SHI Weijun,RUAN Zhouxi,LIN Yanxing,YE Yiyou,TAO Hong,WANG Xiao,CHEN Jinding.Co-expression of BTV-16 four structural proteins and preparation of virus-like particles[J].Chinese Journal of Veterinary Science,2021(1):1-7. |
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| Authors: | HUANG Chaohua HUA Qunyi CAO Chenfu SHI Weijun RUAN Zhouxi LIN Yanxing YE Yiyou TAO Hong WANG Xiao CHEN Jinding |
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| Institution: | (College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Animal and Plant Inspection and Quarantine Technology Center,Shenzhen Customs District P.R.C.,Shenzhen,Guangdong 518045,China) |
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| Abstract: | At present,vaccination is an effective method for the control of bluetongue(BT).As a new vaccine,bluetongue virus-like particles(BTV VLPs)has been a research hotspot.To synthesize BTV-16 VLPs,the vector pFastBac-dual was modified to have four multiple cloning sites(MCS)with independent promoters and named pF4.The VP2,VP5,VP3 and VP7 gene of BTV-16 were inserted into four MCSs of pF4,yielding the quadruple expression vectors pF4-VP2-VP5-VP3-VP7.After transposition,the recombinant bacmid Bacmid-VP2-VP5-VP3-VP7 was obtained.The recombinant insect baculovirus rBac-VP2-VP5-VP3-VP7 co-expressing four BTV-16 proteins(VP2,VP5,VP3 andVP7)was obtained by transfecting Sf9 cells.BTV-16 constructive proteins VP2,VP5,VP3 and VP7 were co-expressed and assembled into BTV-16 VLPs in infected Sf9 cells.BTV-16 VLPs were purified by sucrose density-gradient centrifugation and were observed to be empty and double-shelled under the electron microscope. |
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| Keywords: | bluetongue baculovirus BTV-16 virus-like particles |
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