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Haploid Production from Barley Yellow Dwarf Virus Resistant Clones of Lolium
Authors:J Boppenmeier    S Züchner  B Foroughi-Wehr  
Institution:Dipl.-Ing. agr. J. Boppenmeier;, Dipl.-Ing. agr. S. Züchner, Dr. B. Foroughiwehr, BBA, Institut für Resistenzgenetik, D-8059 Grünbach (Germany, F.R.).
Abstract:The investigation was earned out to obtain double haploid homozygous lines of Lolium. Lolium multiflorurm ssp. gaudini cv. ‘Liwelo’, 44 clones and five different cultivars (cv.) of L. perenne were tested with regard to their anther culture response on a solid medium. Callus production and plant regeneration of the different genotypes were investigated. Within L. m. ssp. gaudini green plants have been obtained. In L. perenne significant genotypical differences in the in vitro reaction could be demonstrated. A high-responding genotype was selected which produced a maximum of 20 % green plants. A variety × temperature interaction was found for the L. perenne varieties ‘Premier’ and ‘Barlenna’. The production of 34 green plants out ot eight clones enables a first analysis to be made of the inheritance of Barley Yellow Dwarf Virus resistance in L. perenne.
Keywords:Lolium multiflorum                        Lolium perenne            BYDV-resistance  anther-culture
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