Effect of parathion on honey bee larvae rearedin vitro |
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| Authors: | Dr Chr Czoppelt Prof Dr H Rembold |
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| Institution: | 1. Max-Planck-Institut für Biochemie, D-8033, Martinsried
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| Abstract: | First instar honey bee larvae (0.2–0.4 mg) from outdoor (summer season) and flight-room colonies (winter season) were reared in an incubator on a semisynthetic diet. Parathion was either dissolved in the diet (0.01–10.0 μg/ml) or topically applied (0–0.5 μg) to the 3rd instar larvae. Increasing doses of parathion caused a delay in larval development and prepupal mortality. The half lethal concentration (LC50) of the insecticide, if applied in the diet, was 0.02 and 0.14 μg/larva, respectively, for summer and for winter larvae. The half lethal dose (LD50) after topical application was 0.18 μg parathion/larva. The utility of rearing honey bee larvaein vitro for evaluation of pesticide toxicity is also discussed. |
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