| PCR及相关技术在鸡球虫种株鉴别方面的应用 |
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| 引用本文： | 李文超,陶建平,沈永林,顾有方,陈会良,彭丽萍.PCR及相关技术在鸡球虫种株鉴别方面的应用[J].动物医学进展,2006,27(9):18-22. |
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| 作者姓名： | 李文超 陶建平 沈永林 顾有方 陈会良 彭丽萍 |
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| 作者单位： | 安徽科技学院动物科学学院,安徽凤阳,233100;扬州大学兽医学院,江苏扬州,225009;南京农业大学动物医学院,江苏南京,210095 |
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| 基金项目： | 安徽科技学院校科研和教改项目;安徽科技学院校科研和教改项目 |
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| 摘 要： | 鸡球虫病是危害养禽业的重大疫病之一,其病原属于艾美耳属(Eimeria)的球虫,世界上公认有7种。过去,其病原的鉴别一直是研究的难点。近年来,由于分子生物学技术,尤其是PCR及其相关技术的迅猛发展及在寄生虫分类中的应用,鸡球虫种、株鉴别方法也由以形态特征和生活史等为标准的传统分类学进入了以核酸为材料的现代分子分类学时代。文章对PCR及其相关技术在鸡球虫种、株鉴别方面的应用做了较为全面的阐述,并分析了其优缺点。
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| 关 键 词： | 球虫 聚合酶链式反应 鸡 |
| 文章编号： | 1007-5038（2006）109-0018-05 |
| 收稿时间： | 2006-03-15 |
| 修稿时间： | 2006年3月15日 |
Application of Techniques Based on PCR in the Differentiation of Eimeria Species and Strain from Chickens |
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| LI Wen-chao,TAO Jian-ping,SHEN Yong-lin,GU You-fang,CHEN Hui-liang,PENG Li-ping.Application of Techniques Based on PCR in the Differentiation of Eimeria Species and Strain from Chickens[J].Progress In Veterinary Medicine,2006,27(9):18-22. |
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| Authors: | LI Wen-chao TAO Jian-ping SHEN Yong-lin GU You-fang CHEN Hui-liang PENG Li-ping |
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| Institution: | 1. College of Animal Science ,Anhui Science and Technology University ,Fengyang,Anhui,233100, China; 2. College of Veterinary Medicine, Yangzhou University , Yangzhou , Jiangsu, 225009, China; 3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing , Jiangsu, 210095, China |
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| Abstract: | Chicken coccidiosis,one of the important diseases,severely endangers poultry industry.Its pathogens belong to Eimeria.In the past,the pathogenic differentiations were very difficult.With the rapid development of molecular biological techology and the application in parasite taxology,the strain differentiation of Eimeria species and strain from chickens entries the molecular biological taxology from the traditional taxology.The review describes systemically the application and progress of molecular biological methods based on PCR in the differentiation of Eimeria species and strain from chickens,and analyzes briefly their advantages and disadvantages. |
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| Keywords: | Eimeria polymerase chain reaction(PCR) chicken |
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