| EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE EMBRYO CULTURE OF TRITICUM AESTIVUM L. - THINOPYRUM INTERMEDIUM ALLEN DISOMIC ADDITION LINES |
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| 作者姓名： | LIHong-mei LIXing-feng GAOJu-rong WANGHong-gang |
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| 作者单位： | [1]IAgronomyCollegeofShandongAgricultureUniversity,Taian271018,China [2]IAgronomyCollegeofShandongAgricultureUniversity,Taian271018,China／／SubcenterofNationalWheatImprovementCenterinTaian,Taian271018,China |
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| 基金项目： | SupportedbytheAgriculturalMinistrySpecialProgramoftheMajorTechnicalResearchonAgricultureStructureAdjustment(No.2003-02-05A) |
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| 摘 要： | An efficient plant regeneration system was developed from the immature embryos of Triticum aestivum L. Thinopyrum intermedium alien disomic addition lines, which resistant to powdery mildew. The protocol was based on a series of experiments involving the callus induction and differentiation. The experiment studied the effects ofmature embryos. We found that the embryembryo size on callus induction and differentiation of the immature embryos. We found that the embryo size is critical for the establishment of embryogenic callus. Immature embryos (0.8 ~ 1.5 ram) showed high ability to produce embryogenie callus capable of regenerating green plants. The medium Murashige and Skoog‘ s (MS) added with 2mg/L 2, 4-diehlo-rophenoxyacetic acid (2, 4-D) gave the best embryogenic callus induction, maintenance and regeneration. The embryogenic callus maintained high regeneration during six subcultures in the callus induction medium. Suitable time ofpartialdesiccation could effectively improve the regeneration capacity of the callus cultured for 3-4 month. Bud green spot and root green spot were observed during the differentiation of callus and the difference between them was described. Regenerated shoots were rooted on half-strength MS medium containing 0.2 mg/L Naphthalene acetic acid (NAA). Plants were successfully transferred to soil and grew well. This efficient plant regeneration system provides a foundation for the study of somaclonal variation of Triticum aestivum L.- Thinopyrum intermedium alien disomic addition lines.
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| 关 键 词： | 小麦 愈伤组织 组织培养 植株再生 培养基 未成熟晶胚 体细胞无性系 |
EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE EMBRYO CULTURE OF TRITICUM AESTIVUM L.- THINOPYRUM INTERMEDIUM ALIEN DISOMIC ADDITION LINES |
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| LIHong-mei LIXing-feng GAOJu-rong WANGHong-gang.EFFICIENT CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE EMBRYO CULTURE OF TRITICUM AESTIVUM L. - THINOPYRUM INTERMEDIUM ALLEN DISOMIC ADDITION LINES[J].Journal of Shandong Agricultural University,2005,36(1):1-8. |
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| Authors: | LI Hong-mei LI Xing-feng GAO Ju-rong WANG Hong-gang |
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| Institution: | 1. Iagronomy College of Shandong Agriculture University, Taian 271018,China
2. Iagronomy College of Shandong Agriculture University, Taian 271018,China;Subcenter of National Wheat Improvement Center in Taian, Taian 271018,China |
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| Abstract: | An efficient plant regeneration system was developed from the immature embryos of Triticum aestivum L. - Thinopyrum intermedium alien disomic addition lines, which resistant to powdery mildew. The protocol was based on a series of experiments involving the callus induction and differentiation. The experiment studied the effects of embryo size on callus induction and differentiation of the immature embryos. We found that the embryo size is critical for the establishment of embryogenic callus. Immature embryos (0.8～1.5 mm) showed high ability to produce embryogenic callus capable of regenerating green plants. The medium Murashige and Skoog's (MS) added with 2mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D) gave the best embryogenic callus induction, maintenance and regeneration. The embryogenic callus maintained high regeneration during six subcultures in the callus induction medium. Suitable time of partial desiccation could effectively improve the regeneration capacity of the callus cultured for 3～4 month.Bud green spot and root green spot were observed during the differentiation of callus and the difference between them was described. Regenerated shoots were rooted on half-strength MS medium containing 0.2 mg/L Naphthalene acetic acid (NAA). Plants were successfully transferred to soil and grew well. This efficient plant regeneration system provides a foundation for the study of somaclonal variation of Triticum aestivum L. - Thinopyrum intermedium alien disomic addition lines. |
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| Keywords: | alien disomic addition lines immature embryos induction differentiation somaclones |
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