| 枯草杆菌β-甘露聚糖酶基因的克隆及表达 |
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| 引用本文： | 毛绍名,章怀云,张学文.枯草杆菌β-甘露聚糖酶基因的克隆及表达[J].农业生物技术学报,2007,15(2):360-361. |
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| 作者姓名： | 毛绍名 章怀云 张学文 |
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| 作者单位： | 1. 中南林业科技大学生命科学与技术学院,长沙,410004
2. 湖南农业大学生物科学技术学院,长沙,410128 |
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| 摘 要： | 以能水解魔芋葡甘聚糖的野生筛选菌种枯草杆菌A33为材料，通过PCR技术从A33基因组中扩增出β-甘露聚糖酶基因编码序列。经过克隆、测序及BLAST比对分析，证实该基因编码β-甘露聚糖酶，属于β-甘露聚糖酶家族中的一员。该基因已注册GenBank（GenBank注册号：DQ269473）。将该基因构建到大肠杆菌表达载体pET-32a并转入大肠杆菌表达系统BL21（DE3），经过诱导获得了此酶的高效表达.
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| 关 键 词： | β-甘露聚糖酶 基因克隆 表达 |
| 文章编号： | 1006-1304（2007）02-0360-02 |
| 收稿时间： | 2006-06-05 |
| 修稿时间： | 2006-07-06 |
Clone and Expression of β-mannanase Gene from Bacillus subtilis |
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| MAO Shao-ming,ZHANG Huai-yun,ZHANG Xue-wen.Clone and Expression of β-mannanase Gene from Bacillus subtilis[J].Journal of Agricultural Biotechnology,2007,15(2):360-361. |
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| Authors: | MAO Shao-ming ZHANG Huai-yun ZHANG Xue-wen |
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| Institution: | 1. College of Life Science and Technology, Central South University of Forestry and Technology, Changshn 410004, China; and Technology, Hunan Agricultural University, Changshn 410128, Cinna |
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| Abstract: | A selected strain of Bacillus subtilis A33 which can hydrolysis the glucomannan of Amorphophallus is used as material to isolate the full-length gene of β-mannanase by PCR. Sequence analysis show this DNA fragment encodes β-mannanase which belongs to the family of mannanase.This Sequence had was submitted in GenBank（GenBank Accession：DQ269473）.To express it in E. coli it is recombinant into the high efficiency expressive vector pET-32a and a recombinant plasmid pET-32a-ManA is constructed and transformed into strain E. coli BL21（DE3）.β-mannanase is expressed efficiently after inducing. |
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