| 大樱桃根癌病原细菌分类地位鉴定 |
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| 引用本文： | 刘保友,张伟,栾炳辉,宫海鹏,盖中帅.大樱桃根癌病原细菌分类地位鉴定[J].湖北农业科学,2009,48(10). |
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| 作者姓名： | 刘保友 张伟 栾炳辉 宫海鹏 盖中帅 |
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| 作者单位： | 山东省烟台市农业科学研究院,山东,烟台,265500 |
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| 基金项目： | 山东省科技攻关项目，烟台市科技攻关项目 |
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| 摘 要： | 从烟台地区采集13份大樱桃根癌病样本,分离纯化出26个菌株,致病性测定表明23个菌株在指示寄主番茄上具有致病性;进一步经生理生化反应、碳源利用、选择性培养基等试验鉴定出16个根癌病菌菌株为Agrobacterium tumefaciens生物型2,占69.57%,为优势种群,其余7个菌株为生物型1.对大樱桃根癌病菌分类地位的确定为今后病原菌的系统研究和病害防治提供了理论基础.
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| 关 键 词： | 大樱桃 根癌 病原细菌 分类地位 鉴定 |
Taxonomic Status Study of Agrobacterium tumefaciens Isolated from Sweet Cherry |
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| LIU Bao-you,ZHANG Wei,LUAN Bing-hui,GONG Hai-peng,GAI Zhong-shuai.Taxonomic Status Study of Agrobacterium tumefaciens Isolated from Sweet Cherry[J].Hubei Agricultural Sciences,2009,48(10). |
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| Authors: | LIU Bao-you ZHANG Wei LUAN Bing-hui GONG Hai-peng GAI Zhong-shuai |
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| Abstract: | 26 strains was obtained from 13 samples of crown gall on sweet cherry in Yantai. 23 strains could form crown gall tomato.These strains were identified further based on reactions to standard biochemical and physiological tests,by carbon utilization and by selective media for identification. Among 23 strain,16 strains were Agrobacterium tumefaciens biotype 2,which made 69.57% of total amount. The other 7 strains were A. tumefaciens biotype 1. The result indicated that the dominant biotype of A. tumefaciens was biotype 2 in Yantai. The establishment of A. tumefaciens causing crown gall disease of sweet cherry laid the foundations for systematic study on pathogenic A. tumefaciens and disease control. |
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| Keywords: | sweet cherry crown gall pathogenic bacteria taxonomic status identification |
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