| 术前饥饿处理对马氏珠母贝血淋巴免疫机能的影响 |
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| 引用本文： | 李琼珍,罗帮,张立,王有基.术前饥饿处理对马氏珠母贝血淋巴免疫机能的影响[J].上海海洋大学学报,2014,23(4):505-512. |
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| 作者姓名： | 李琼珍 罗帮 张立 王有基 |
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| 作者单位： | 广西壮族自治区水产科学研究院,广西壮族自治区水产科学研究院,上海海洋大学,广西壮族自治区水产科学研究院 |
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| 基金项目： | 广西水产畜牧兽医局渔业处2013年项目资助; 现代农业产业技术体系建设专项资金资助 |
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| 摘 要： | 在室内养殖条件下研究了术前限食处理对马氏珠母贝血淋巴细胞密度、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LSZ)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性的影响。实验设置每天投喂一次和两天投喂一次两个处理组,每天投喂两次为对照组,分别在实验开始和第1、2、3和4周取样检测血淋巴免疫功能。结果显示:限食1周后,马氏珠母贝血细胞密度、SOD、CAT、LSZ、ACP和AKP等酶活性均随限食程度的增加呈下降趋势,2个限食处理组的免疫参数较对照组明显下降,并表现出显著性差异(P0.05),两天投喂一次处理组的各项免疫机能均显著低于对照组,且这种趋势一直延续至第4周。据此推断,限食可以显著降低插核手术贝的生理机能和免疫活性,为马氏珠母贝育珠术前处理提供理论依据。
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| 关 键 词： | 马氏珠母贝 术前处理 免疫机能 血淋巴 饥饿 |
| 收稿时间： | 2014/2/11 0:00:00 |
| 修稿时间： | 4/6/2014 12:00:00 AM |
Effects of starvation on immune function of hemolymph in pearl oyster Pinctada martensii during pre-operation |
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| LI Qiong-zhen,LUO Bang,ZHANG Li and WANG You-ji.Effects of starvation on immune function of hemolymph in pearl oyster Pinctada martensii during pre-operation[J].Journal of Shanghai Ocean University,2014,23(4):505-512. |
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| Authors: | LI Qiong-zhen LUO Bang ZHANG Li and WANG You-ji |
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| Institution: | Guangxi Institute of Fisheries,Guangxi Institute of Fisheries,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306,Guangxi Institute of Fisheries |
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| Abstract: | Effects of starvation on immune activity of hemolymph in Pinctada martensii during pre-operating were investigated under laboratory condition, including total hemocyte counts (THC), superoxide dismutase (SOD), catalase (CAT), lysozyme (LSZ), acid phosphatase (ACP) and alkaline phosphatase (AKP). There were three treatments in the experiments, feeding once daily, feeding once two days, and feeding twice daily as a control. Hemolymph was sampled at the beginning of the experiment, and 1, 2, 3, 4 week to evaluate the immune functions. The results showed THC, SOD, CAT, LSZ, ACP and AKP activities decreased with the increase of fasting degree after one week fasting, immune parameters of the two fasting treatments significantly reduced compared with the control group, and displayed significant difference (P<0.05). All immune parameters in feeding once two days treatment were lower than the control treatment, and the trend lasted for four weeks. Therefore, starvation can significantly reduce the physiological functions and immune activities, and this paper can provide theoretical basis for the pre-operation of pearl culture. |
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| Keywords: | Pinctada martensii stravation hemolymph pre-operation immune functions |
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