| CRH、UⅠ和UⅡ基因在斑马鱼发育早期的表达谱 |
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| 引用本文： | 罗蕾,陈阿琴,吕为群.CRH、UⅠ和UⅡ基因在斑马鱼发育早期的表达谱[J].上海海洋大学学报,2014,23(4):481-486. |
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| 作者姓名： | 罗蕾 陈阿琴 吕为群 |
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| 作者单位： | 上海海洋大学水产与生命学院,上海海洋大学水产与生命学院,上海海洋大学水产与生命学院 |
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| 基金项目： | 国家自然科学基金(31072228、41376134)；高等教育博士点基金(20113104110002)；上海高校水产学一流学科建设和知识服务平台项目资助；上海市研究生优秀论文培育计划 |
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| 摘 要： | 促肾上腺皮质激素释放激素(Corticotropin-releasing hormone,CRH)、尾加压素Ⅰ(UrotensinⅠ,UⅠ)和尾加压素Ⅱ(UrotensinⅡ,UⅡ)广泛存在于脊椎动物中。在鱼类中,它们在渗透压调节、繁殖、营养代谢以及应激反应中发挥着重要作用。本研究中,通过收集0、1.5、3.7、5.3、8、10、16、24、48、72、96、120和144 hpf(hours post-fertilization)的斑马鱼早期发育样品,运用荧光定量PCR技术进行检测发现CRH、UⅠ、UⅡα和UⅡβmRNA从0 hpf开始存在表达,而且CRH、UⅠ、UⅡα和UⅡβ的表达量随发育而逐渐增加,并分别在120 hpf或144 hpf达到最高水平。相关性分析揭示在斑马鱼早期发育过程中,CRH、UⅠ、UⅡα和UⅡβmRNA的表达水平两两之间呈现极显著正相关,这表明CRH、UⅠ、UⅡα和UⅡβ在斑马鱼早期发育过程中关系紧密并且相互合作发挥作用。
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| 关 键 词： | 斑马鱼 个体发育 荧光定量PCR 基因表达 促肾上腺皮质激素释放激素 尾加压素 |
| 收稿时间： | 2014/1/14 0:00:00 |
| 修稿时间： | 2014/3/26 0:00:00 |
Dynamic expression pattern of CRH, UI and UII in zebrafish during early development |
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| LUO Lei,CHEN A-qin and L&#; Wei-qun.Dynamic expression pattern of CRH, UI and UII in zebrafish during early development[J].Journal of Shanghai Ocean University,2014,23(4):481-486. |
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| Authors: | LUO Lei CHEN A-qin and L&#; Wei-qun |
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| Institution: | SLaboratorySofSAdaptiveSPhysiology,CollegeSofSFisheriesSandSLifeSScienceShanghaiSOceanUniversity,Shanghai,SLaboratorySofSAdaptiveSPhysiology,CollegeSofSFisheriesSandSLifeSScienceShanghaiSOceanUniversity,Shanghai,SLaboratorySofSAdaptiveSPhysiology,CollegeSofSFisheriesSandSLifeSScienceShanghaiSOceanUniversity,Shanghai |
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| Abstract: | Corticotropin-releasing hormone （ CRH）, Urotensin I （ U I ） and Urotensin Ⅱ （ U Ⅱ ） have been found throughout vertebrate species from fish to human. In fish, these peptides appear to be involved in osmoregulation, reproduction, nutritional behavior and stress responses. Embryos and larvae were collected at 0,1.5,3. 7,5. 3,8,10, 16,24,48,72,96,120 and 144 hpf （ hours post-fertilization）. Three biological replicate pools （n = 50 larvae per pool ） were analyzed for each developmental time point. Real-time quantitative PCR results showed that the mRNA coding CRH, U1, UⅡα and UⅡβ was present from 0 hpf, suggesting a maternal function. The relative amounts of CRH, U1, UⅡα and UⅡβ mRNA during the early development of zebrafish gradually increased and reached the highest level at 144 hpf except UⅡα at 120 hpf. Highly significant and positive correlations between each pair of CRH, UI, UⅡα and UⅡβ mRNA expression levels were found. It suggested that these genes were closely linked and cooperated to support a developmental function. |
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| Keywords: | Zebrafish Development Real-time Quantitative PCR Gene expression Corticotropin releasing hormone Urotensins |
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