| 三角梅属5个品种的花粉活力及柱头可授性比较分析 |
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| 引用本文： | 杜学林,刘悦明,黄子锋,刘慧雅,胡智仁,李志美,王凤兰.三角梅属5个品种的花粉活力及柱头可授性比较分析[J].热带作物学报,2022,43(7):1459-1466. |
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| 作者姓名： | 杜学林 刘悦明 黄子锋 刘慧雅 胡智仁 李志美 王凤兰 |
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| 作者单位： | 1.仲恺农业工程学院, 广东广州 5102252.广州市林业和园林科学研究院, 广东广州 5104053.东莞市农业科学研究中心,广东东莞 5230834.广州市花都区仲花现代农业研究院, 广东广州 510800 |
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| 基金项目： | 2021年省级农业科技创新及推广项目(2021KJ121) |
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| 摘 要： | 为探究三角梅属植物不同品种的花粉活力和柱头可授性,从而提高其杂交效率。以‘伊娃夫人’（Bougainvillea glabra ‘Mrs Eva’）、‘小叶紫’（B. glabra ‘Sao paulo’）、‘伊娃白’（B. glabra ‘Mrs Eva White’）、‘加州黄金’（B. × buttiana ‘California Gold’）和‘橙红’（B. × buttiana ‘Afterglow’）等5个三角梅品种为试材,采用FDA、液体培养基萌发法检测5个品种的花粉生活力,探究不同保存方法对其花粉活力的影响;利用联苯胺-过氧化氢法对5个品种的柱头可授性进行比较分析。结果表明：（1）0.01%FDA染色液均能使5个三角梅品种染色,其中,‘伊娃白’花粉活力最高,花粉活力为27.16%;其次是‘伊娃夫人’和‘小叶紫’,分别为13.98%和11.86%;活力最低的是‘橙红’和‘加州黄金’,分别为7.17%和4.01%。（2）不同保存条件下,5个三角梅品种的花粉活力存在显著差异。28℃干燥保存条件下,各品种花粉活力大小顺序为‘小叶紫’>‘伊娃夫人’>‘伊娃白’>‘橙红’>‘加州黄金’;4℃冰箱保存条件下,各品种花粉活力大小顺序为‘伊娃夫人’>‘伊娃白’>‘小叶紫’>‘加州黄金’>‘橙红’。（3）通过液体培养基培养12 h后5个三角梅品种的花粉萌发率均较低,其中‘小叶紫’萌发率相对较高,为4.50%;其次是‘伊娃白’和‘伊娃夫人’,分别为2.78%和2.51%;‘加州黄金’和‘橙红’萌发率最低,分别为0.98%和0.08%。（4）5个三角梅品种的柱头可授性差异明显,其中‘小叶紫’和‘伊娃夫人’柱头可授性较好,其次是‘伊娃白’,而‘加州黄金’和‘橙红’柱头可授性均较低。综上,‘小叶紫’‘伊娃夫人’和‘伊娃白’3个三角梅品种适合作为杂交育种中的亲本材料。
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| 关 键 词： | 三角梅属 花粉活力 柱头可授性 杂交育种 |
| 收稿时间： | 2021-11-29 |
Comparative Analysis of Pollen Viability and Stigma Receptivity of Five Bougainvillea Cultivars |
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| DU Xuelin,LIU Yueming,HUANG Zifeng,LIU Huiya,HU Zhiren,LI Zhimei,WANG Fenglan.Comparative Analysis of Pollen Viability and Stigma Receptivity of Five Bougainvillea Cultivars[J].Chinese Journal of Tropical Crops,2022,43(7):1459-1466. |
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| Authors: | DU Xuelin LIU Yueming HUANG Zifeng LIU Huiya HU Zhiren LI Zhimei WANG Fenglan |
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| Institution: | 1. Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China2. Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou, Guangdong 510405, China3. Dongguan Agricultural Science Research Center, Dongguan, Guangdong 523083, China4. Zhonghua Modern Agriculture Research Institute, Huadu District, Guangzhou, Guangdong 510800, China |
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| Abstract: | The purpose of this study was to investigate the pollen viability and stigma receptivity of different varieties of Bougainvillea so as to improve the hybridization efficiency. Using five Bougainvillea varieties including B. glabra ‘Mrs Eva’, B. glabra ‘Sao paulo’, B. glabra ‘Mrs Eva White’, B. × buttiana ‘California Gold’ and B. × buttiana ‘Afterglow’ as test materials, the pollen viability was tested by FDA and liquid culture medium methods, and effects of different preservation methods on pollen viability were explored. The stigma receptivity of five Bougainvillea cultivars was analyzed by benzidine- hydrogen peroxide method. The results showed that: (1) 0.01% FDA staining solution could stain all varieties. The pollen viability of B. glabra ‘Mrs Eva White’ was the highest (27.16%), followed by B. glabra ‘Mrs Eva’ and B. glabra ‘Sao paulo’, which were 13.98% and 11.86%, respectively. The lowest pollen viability of B. × buttiana ‘Afterglow’ and B. × buttiana ‘California Gold’ were 7.17% and 4.01%, respectively. (2) Pollen viability of five cultivars differed significantly under different storage conditions. Under the condition of drying and preservation at 28℃, the order of vigor of each variety was B. glabra ‘Sao paulo’ > B. glabra ‘Mrs Eva’ > B. glabra ‘Mrs Eva White’ > B. × buttiana ‘Afterglow’ > B. × buttiana ‘California Gold’. Under the condition of 4℃ refrigerator, the order of vitality of each variety was B. glabra ‘Mrs Eva’ > B. glabra ‘Mrs Eva White’ > B. glabra ‘Sao paulo’ > B. × buttiana ‘California Gold’ > B. × buttiana ‘Afterglow’. (3) The pollen germination rates of five varieties were all low after 12 hours of culture in liquid medium. The pollen germination rate of B. glabra ‘Sao paulo’ was relatively high (4.50%), B. glabra ‘Mrs Eva White’ and B. glabra ‘Mrs Eva’ followed with 2.78% and 2.51% respectively. B. × buttiana ‘California Gold’ and B. × buttiana ‘Afterglow’ had the lowest germination rates (0.98% and 0.08%, respectively). (4) There were significant differences in stigma acceptability among five cultivars. The stigma acceptability of B. glabra ‘Sao paulo’ and B. glabra ‘Mrs Eva’ was better, followed by B. glabra ‘Mrs Eva White’, but the stigma acceptability of B. × buttiana ‘California Gold’ and B. × buttiana ‘Afterglow’ was lower. In conclusion, three Bougainvillea varieties, such as B. glabra ‘Sao paulo’, B. glabra ‘Mrs Eva’ and B. glabra ‘Mrs Eva White’ are suitable as parent materials in cross breeding. |
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| Keywords: | Bougainvillea pollen vitality stigma receptivity cross breeding |
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