| 生防枯草芽孢杆菌HMB19198发酵培养基的筛选及优化 |
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| 引用本文： | 曲远航,郭庆港,李社增,宣立锋,张晓云,马平.生防枯草芽孢杆菌HMB19198发酵培养基的筛选及优化[J].农药学学报,2022,24(3):509-519. |
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| 作者姓名： | 曲远航 郭庆港 李社增 宣立锋 张晓云 马平 |
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| 作者单位： | 1.河北省农林科学院 植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业农村部华北北部作物有害生物综合治理重点实验室，河北 保定 071000 |
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| 基金项目： | 河北省重点研发计划(19226510D)；河北省农林科学院现代农业科技创新工程(2019-1-1-7). |
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| 摘 要： | 枯草芽孢杆菌Bacillus subtilis HMB19198菌株能有效防治番茄灰霉病，脂肽类抗生素泛革素(fengycin)是该菌株产生的主要抑菌活性物质。为提高HMB19198菌株的发酵水平，本研究以活芽孢浓度为检测指标，通过对8种常用工业培养基进行筛选，获得了利于HMB19198菌株芽孢形成的基础培养基。采用单因素试验筛选HMB19198菌株芽孢产量较高的碳源和氮源组合，结果表明，以可溶性玉米淀粉和糖蜜作为碳源、以花生饼浸粉和蛋白胨作为氮源时，有利于HMB19198菌株芽孢的形成。进一步运用Plackett-Burman及响应曲面分析相结合的方法，对碳源物质玉米淀粉和糖蜜，氮源物质花生饼浸粉和蛋白胨，以及无机盐K2HPO4?3H2O和MgSO4?7H2O的适宜浓度进行了优化。优化后发酵培养基配方中各成分的质量浓度分别为:可溶性玉米淀粉67.0 g/L、糖蜜14.1 g/L、花生饼浸粉41.6 g/L、蛋白胨10 g/L、K2HPO4?3H2O 9.2 g/L以及MgSO4?7H2O 1.5 g/L。在优化培养基中，HMB19198菌株发酵液的芽孢浓度可达到6.92 × 109 个/mL；同基础培养基相比，采用优化后的培养基，菌株发酵周期缩短了40%，发酵液中芽孢浓度提高了107%，泛革素浓度提高了39.4%。
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| 关 键 词： | 枯草芽孢杆菌 发酵液 芽孢 培养基优化 响应曲面分析 泛革素 |
| 收稿时间： | 2021-10-22 |
Screening and Optimization of Bacillus subtilis HMB19198 fermentation medium |
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| Institution: | 1.Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, Hebei Province, China2.Institute of Shijiazhuang Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050061, China |
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| Abstract: | Bacillus subtilis strain HMB19198 is a promising biocontrol agent for tomato gray mold. Fengycin, a kind of lipopeptide, plays an important role in strain HMB19198 suppressing tomato gray mold. To improve the fermentation level of strain HMB19198, the basic medium benefiting for the sporulation of strain HMB19198 was selected from eight kinds of industrial mediums. The carbon and nitrogen sources suitable for sporulation of strain HMB19198 were screened by a single factor test. Results showed that soluble corn starch and molasses as carbon sources, peanut cake powder, and peptone as nitrogen sources were conducive to the sporulation of strain HMB19198. The Plackett-Burman analysis and response surface analysis were used to optimize the concentrations of soluble corn starch, molasses, peanut cake powder, peptone, K2HPO4?3H2O, and MgSO4?7H2O in the medium. The optimized medium included 67.0 g/L soluble corn starch, 14.1 g/L molasses, 41.6 g/L peanut cake powder, 10 g/L peptone, 9.2 g/L K2HPO4?3H2O and 1.5 g/L MgSO4?7H2O. In the optimized medium, the spore concentration of strain HMB19198 reached 6.92 × 109 spore/mL. Compared with the basic medium, using the optimized medium, the fermentation period of the strain was shortened by 40%, the spore concentration in the fermentation broth was increased by 107%, and the fengycin concentration was increased by 39.4%. |
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