| 氮磷钾配施对北苍术产量、品质及其相关酶基因表达的影响 |
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| 引用本文： | 冯禹壮,徐伟,肖春萍,翁丽丽,孙金,石婷婷.氮磷钾配施对北苍术产量、品质及其相关酶基因表达的影响[J].植物营养与肥料学报,2022,28(2):302-312. |
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| 作者姓名： | 冯禹壮 徐伟 肖春萍 翁丽丽 孙金 石婷婷 |
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| 作者单位： | 长春中医药大学药学院，吉林长春 130117 |
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| 基金项目： | 国家自然科学基金项目(81803649)；吉林省科技厅科技项目(20191102035YY)；吉林省中医药管理局科技项目(2020047)；2021年吉林省省级大学生创新创业训练计划项目。 |
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| 摘 要： | 目的]研究氮磷钾配施对北苍术的产量、苍术素含量及乙酰辅酶A羧化酶(ACC)活性和基因表达量的影响,探讨氮磷钾配施对北苍术药材产量增加、质量形成的影响及其机制,对提高北苍术药材产量及质量有重要的现实意义.方法]以一年生北苍术为试验材料,采用L9(34)?正交设计,设置10个不同氮、磷、钾用量处理的田间试验,在营养生长...
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| 关 键 词： | 北苍术 肥料配施 苍术素 乙酰辅酶A羧化酶(ACC) 基因表达 |
| 收稿时间： | 2021-07-26 |
Effects of combination of nitrogen,phosphorus and potassium on the yield,quality and the related enzyme gene expression of Atractylodes chinensis |
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| FENG Yu-zhuang,XU Wei,XIAO Chun-ping,WENG Li-li,SUN Jin,SHI Ting-ting.Effects of combination of nitrogen,phosphorus and potassium on the yield,quality and the related enzyme gene expression of Atractylodes chinensis[J].Plant Nutrition and Fertilizer Science,2022,28(2):302-312. |
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| Authors: | FENG Yu-zhuang XU Wei XIAO Chun-ping WENG Li-li SUN Jin SHI Ting-ting |
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| Institution: | School of Pharmaceutical Sciences Changchun University of Chinese Medicine, Changchun, Jilin130117, China |
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| Abstract: | 【Objectives】 The suitable combination of nitrogen (N), phosphorus (P) and potassium (K) fertilizers was studied from the yield, atrctylodin content and the activity and gene expression of acetyl coenzyme A carboxylase (ACC) of A. chinensis, so to propose a scientific fertilizer combination for improving the productivity and quality of A. chinensis. 【Methods】 A field experiment including 10 combined applications of N, P and K fertilizers was set up following a L9(34) design, with one-year-old Atractylodes chinensis as experimental material. The fresh weight of aboveground part, rhizome, and fibrous root of A. chinensis were weighed at vegetative growth, flowering, early fruiting, full fruiting and withering stages. The plant samples were collected at the same time for determination of atrctylodin content by HPLC method, the ACC activity in rhizome by double antibody ELISA method, the relative expression level of ACC gene using real-time PCR. Spearman correlation analysis was used to determine the molecular regulation mechanism of atrctylodin synthesis. 【Results】 All the NPK combined applications promoted the growth of aboveground part, rhizome and fibrous root of the crop. The most significant effects were observed in T3 (N1P3K3) and T6 (N2P3K1), the fresh weight of aboveground part were respectively 1.52 and 1.47 times that from N0P0K0 at full fruiting period, the fresh weight of underground rhizome were 2.08 and 2.09 times of that from N0P0K0 at withering period, and the fresh weight of fibrous roots were 1.30 and 1.41 times of that from N0P0K0 at withering period. Compared with N0P0K0, T3 and T6 increased atrctylodin by 33% and 61% at withering period. Moreover, according to the dynamic accumulation characteristics of atrctylodin in different growth stages, it was speculated that the most important period for atrctylodin accumulation was the withering period to the next year before emergence. According to the results of correlation analysis, the correlation coefficients between atrctylodin content and ACC enzyme activity, ACC gene expression were 0.66 and 0.583, respectively. The correlation coefficient between ACC enzyme activity and ACC gene expression was 0.839, which reached extremely significant positive correlation (P<0.01). Treatment of high P with low and medium N rates improved the gene expression of ACC and enhanced the ACC activity thereby increased the accumulation of atrctylodin. While high level of N and K inhibited the gene expression of ACC, thereby decreased the accumulation of atrctylodin. 【Conclusions】 High level of P fertilizer (225 kg/hm2), combined with appropriate level of N (90 kg/hm2) and K fertilizer (315 kg/hm2) or P (225 kg/hm2), N (180 kg/hm2) and K fertilizer (105 kg/hm2) are beneficial to the yield of A. chinensis, which will stimulate the gene expression, enhance ACC activity, and promote the synthesis and accumulation of atrctylodin. |
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