| 应用多酚–叶绿素仪监测棉花氮素营养状况研究 |
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| 引用本文： | 殷星,侯振安,冶军,闵伟,刘凯,王方斌,廖欢,甘浩天,刘少华,孙嘉璘.应用多酚–叶绿素仪监测棉花氮素营养状况研究[J].植物营养与肥料学报,2021,27(7):1198-1212. |
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| 作者姓名： | 殷星 侯振安 冶军 闵伟 刘凯 王方斌 廖欢 甘浩天 刘少华 孙嘉璘 |
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| 作者单位： | 石河子大学农学院农业资源与环境系，新疆石河子 832003 |
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| 基金项目： | 国家重点研发计划 (2017YFD0200100，2018YFD0800800)；兵团中青年科技创新领军人才计划 (2016BC001) |
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| 摘 要： | 【目的】 通过田间试验，研究使用多酚–叶绿素仪对棉花进行快速无损氮素营养诊断适宜的指标。 【方法】 田间试验在新疆石河子市进行，设3个施氮处理，分别为施纯N 0、180和240 kg/hm2，分别用N0、N180和N240表示。所有氮肥分5次随滴灌施入，每次施肥后3天，利用多酚–叶绿素仪 (Dualex-4) 和SPAD叶绿素仪分别测定20株棉花叶片的氮平衡指数 (NBI)、Chl值和SPAD值，同步采样测定棉花叶片全氮含量，及0—20 cm、0—40 cm和0—60 cm土层硝态氮含量。 【结果】 随着施氮量的增加棉花叶片全氮含量和土壤硝态氮含量均显著增加。其中，0—40 cm土层硝态氮含量与棉花叶片全氮含量关系最密切。增加氮肥施用量，棉花叶片氮素营养诊断指标NBI、Chl值和SPAD值均显著增加。棉花叶片NBI、Chl和SPAD与叶片全氮含量均呈极显著正相关关系，且相关系数 (r) 均达到0.8以上。相关性模型校验结果表明，棉花叶片全氮含量实测值与预测值的平均相对误差 (RE) 分别为–4.0% (NBI)、–3.1% (Chl) 和–5.7% (SAPD)。其中，氮平衡指数 (NBI) 模型对棉花叶片全氮含量的预测精度最高，与实测值的相关系数达到了0.9143，平均绝对百分比误差 (MAPE) 为6.91%；标准均方根误差 (nRMSE) 为8.21%。棉花叶片NBI、Chl和SPAD与土壤硝态氮的模型决定系数表现为NBI > Chl > SPAD。模型校验分析表明，NBI模型与0—40 cm土层硝态氮实测含量的相关性最高，相关系数为0.9116，预测值与实测值的MAPE和nRMSE分别为14.11%和17.88%。 【结论】 应用多酚–叶绿素仪监测棉花氮素营养，氮平衡指数 (NBI) 与棉花叶片氮含量和0—40 cm土层硝态氮含量的相关性最高，预测值与实测值的误差仅为6.91%和14.11%，可以满足膜下滴灌条件下棉花氮素营养的快速诊断需求。
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| 关 键 词： | 多酚–叶绿素仪 氮素平衡指数 叶绿素 SPAD 土壤硝态氮含量 棉花叶片全氮含量 模型预测精度 |
| 收稿时间： | 2020-12-23 |
Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status |
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| YIN Xing,HOU Zhen-an,YE Jun,MIN Wei,LIU Kai,WANG Fang-bin,LIAO Huan,GAN Hao-tian,LIU Shao-hua,SUN Jia-lin.Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status[J].Plant Nutrition and Fertilizer Science,2021,27(7):1198-1212. |
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| Authors: | YIN Xing HOU Zhen-an YE Jun MIN Wei LIU Kai WANG Fang-bin LIAO Huan GAN Hao-tian LIU Shao-hua SUN Jia-lin |
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| Institution: | Department of Resources and Environmental Science, Colloge of Agronomy, Shihezi University, Shihezi, Xinjiang 832003, China |
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| Abstract: | 【Objectives】 We studied the suitable parameter in diagnosing cotton N nutrition using the polyphenol-chlorophyll meter in the field. 【Methods】 A field experiment was set up in Shihezi, Xinjiang, with three N application rates: N 0, 180, and 240 kg/hm2, expressed as N0, N180 and N240. The N fertilizer treatments were topdressed 5 times with drip irrigation. Nitrogen balance index (NBI), Chl, and SPAD values of 20 cotton plants leaves were measured on the third day after topdressing, using both Polyphenol-chlorophyll meter (Dualex-4) and SPAD chlorophyll meter. The total N content of cotton leaves and the nitrate-nitrogen content in 0–20 cm, 0–40 cm and 0–60 cm soil layers were determined simultaneously. 【Results】 With increasing N applicationrate, the total N content of cotton leaves and soil nitrate-nitrogen content increased significantly. The soil NO3–-N content in the 0–40 cm soil layer had the closest relationship with the total N content of cotton leaves. The NBI, Chl, and SPAD values of cotton leaves significantly increased with increasing N application rate, and were positively correlated with the total N content of cotton leaves. The correlation coefficients were all above 0.8. The average relative error (RE) of the measured and predicted values of leaf total N content was –4.0% (NBI), –3.1% (Chl), and –5.7% (SAPD), respectively. The NBI exhibited the highest prediction accuracy for leaf N content, with an r-value of 0.9143, average absolute percentage error (MAPE) of 6.91%, and the standard root mean square error (nRMSE) was 8.21%. The model determination coefficient (R2) of the diagnostic index with soil NO3–-N content was in the descending order NBI > Chl > SPAD. The NBI was the most sensitive to NO3–-N content in 0–40 cm soil layer, with r-value of 0.9116, MAPE of 14.11%, and nRMSE of 17.88%. 【Conclusions】 When using the polyphenol-chlorophyll meter to diagnose N nutrition, we find that the N balance index (NBI) is sensitive and accurate in reflecting cotton leaf N content and the NO3–-N content in 0–40 cm soil layer. The average absolute percentage error (MAPE) between the predicted and measured values is as low as 6.91% and 14.11%, respectively, which could meet the requirement for rapid diagnosis of cotton N nutrition. |
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