| 磷对盐碱胁迫下棉花离子平衡及相关调控基因的影响 |
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| 引用本文： | 孙嘉璘,侯振安.磷对盐碱胁迫下棉花离子平衡及相关调控基因的影响[J].植物营养与肥料学报,2022,28(4):643-653. |
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| 作者姓名： | 孙嘉璘 侯振安 |
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| 作者单位： | 石河子大学农学院农业资源与环境系，新疆石河子 832003 |
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| 基金项目： | 国家自然科学基金项目(21660594)。 |
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| 摘 要： | 目的]盐胁迫是影响棉花生长的主要非生物胁迫之一,合理施肥促进盐胁迫下作物对养分离子的吸收,是提高作物耐盐性的重要途径.研究施磷随盐胁迫下棉花离子组的响应特征及Na+转运相关基因表达的变化,探讨磷对棉花耐盐性机制.方法]采用盆栽试验,设置盐胁迫(NaCl)和碱胁迫(NaHCO3+Na2CO3)2个逆境处理,每个逆境下...
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| 关 键 词： | 盐碱胁迫 磷 棉花 离子稳态 Na+转运相关基因 |
| 收稿时间： | 2021-06-22 |
Effect of phosphorus on ionic balance and expression of related regulatory genes of cotton under salt and alkali stresses |
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| SUN Jia-lin,HOU Zhen-an.Effect of phosphorus on ionic balance and expression of related regulatory genes of cotton under salt and alkali stresses[J].Plant Nutrition and Fertilizer Science,2022,28(4):643-653. |
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| Authors: | SUN Jia-lin HOU Zhen-an |
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| Institution: | Department of Agricultural Resources and Environment, Agricultural College, Shihezi University, Shihezi, Xinjiang 832003, China |
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| Abstract: | 【Objectives】 Salt stress is one of the major abiotic stresses affecting the growth of cotton in Xinjiang, and proper fertilization for efficient uptake of nutrient ions under salt stress is an important way to improve salt tolerance in crops. We studied effect of phosphorus on cotton salinity tolerance via the ionome response characteristics and the expression of Na+ transport-related genes. 【Methods】 A cotton pot experiment was conducted, and the stress treatments were prepared by adding NaCl and NaHCO3 + Na2CO3 as salt and base stress treatments, respectively. Under each stress condition, cotton plants were sprayed with 0 (P0), 0.5% (P0.5) and 0.75% (P0.75) of phosphorus fertilizer at three-leaf-stage while the cotton in normal soil was sprayed with water (control). Cotton growth (biomass, root length, root surface area) and physiological indicators of adversity (relative permeability of the plasma membrane, malondialdehyde) were measured to study the effects of phosphorus on salinity tolerance in cotton. The ion content of cotton organs (roots, stems and leaves) was analyzed to elucidate the response characteristics of the cotton ionome. The relative expression levels of GhSOS1 (Na+ transport regulation related gene), GhNHX1 (Na+ compartmental gene), GhAKT1 (K+ transport gene) and GhVP1 (H+-translocating pyrophosphatases gene) in leaves were determined. 【Results】 Compared with P0, P0.5 and P0.75 treatments increased relative biomass of cotton by 23.8% and 34.7% under salt stress, and by 17.3% and 21.1% under alkali stress. Compared with P0 treatment, P0.75 treatment increased cotton root length and root surface area by 74.6% and 49.4% under salt stress, respectively. However, spraying P had no significant effect on cotton root length under alkali stress. The relative permeability of plasma membrane and the content of malondialdehyde in cotton leaves were significantly reduced by spraying phosphorus, and the effect of alleviating salt stress was greater than that of alkaline stress. Under salt and alkali stress, however, the decrease of Na content and the increase of Cu and Si content under salt stress were greater than those under alkali stress. In cotton roots, only Na content was significantly reduced by spraying phosphorus under salt stress, while the content of Ca, Zn, P, and Fe significantly increased, and the content of other ions did not change under salt and alkali stress. K/Na in various organs of cotton increased significantly with the increase of phosphorus levels. This indicated that the ion group of cotton leaves changed in a similar trend under different saline stresses, but the promotion effect of salt stress on ion root uptake in cotton was greater than that of alkali stress. Under salt and alkali stresses, the application of phosphorus significantly increased the relative expression of GhSOS1, GhNHX1, GhAKT1 and GhVP1, which may promote Na+ efflux and compartmentalization in cotton leaves, and the synthesis of tonoplast H+-transport pyrophosphatase. Under alkaline stress, it may greatly poromote K+ transport, which was significantly greater than that under salt stress. 【Conclusions】 Phosphorus spray on leaves can promote the growth of cotton under salt stress, up-regulate the expression of genes related to Na+ transport regulation, reduce leaf Na content, promote nutrient ion uptake and transport, maintain ion homeostasis, and thus improve salt tolerance in cotton. However, it has less alleviating effect on alkali stress. |
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