| 韭菜中腐霉利的残留检测及长期膳食暴露评估 |
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| 引用本文： | 周勇,朴秀英,廖先骏,刘佳,朱航,马海昊,周小毛,李富根.韭菜中腐霉利的残留检测及长期膳食暴露评估[J].农药学学报,2021,23(2):373-379. |
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| 作者姓名： | 周勇 朴秀英 廖先骏 刘佳 朱航 马海昊 周小毛 李富根 |
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| 作者单位： | 1.湖南省农业科学院 湖南省农业生物技术研究所，长沙 410125 |
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| 基金项目： | 国家重点研发计划 (2016YFD0200208) |
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| 摘 要： | 建立了一种简便、高效的QuEChERS前处理结合高效液相色谱-串联质谱 (HPLC-MS/MS) 检测韭菜中腐霉利残留量的方法。于2019年在中国黑龙江、辽宁、河南、山东、安徽、湖南、广西及福建8地开展了田间规范残留试验，研究了腐霉利在韭菜中的残留风险，并进行了韭菜中腐霉利残留的长期膳食暴露评估。样品采用乙腈提取，N-丙基乙二胺 (PSA) 和石墨化碳黑 (GCB) 净化，HPLC-MS/MS检测，外标法定量。结果表明:在添加水平为0.02~20 mg/kg范围内，腐霉利在韭菜中的回收率为74%~98%，相对标准偏差 (RSD) 为4.6%~9.9%，定量限 (LOQ) 为0.02 mg/kg。50%腐霉利可湿性粉剂以有效成分450 g/hm2的剂量于韭菜灰霉病发病初期或前期施药1次，施药后间隔30 d采样，腐霉利在韭菜中的最终残留量为0.04~1.06 mg/kg。长期膳食暴露评估结果显示:腐霉利的国家估算每日摄入量 (NEDI) 为2.5562 mg，风险商值 (RQ) 为40.6%，表明腐霉利在韭菜中的长期膳食摄入风险较低。
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| 关 键 词： | 韭菜 腐霉利 残留 膳食暴露 风险评估 高效液相色谱-串联质谱 QuEChERS |
| 收稿时间： | 2020-05-12 |
Determination and chronic dietary exposure assessment of procymidone residue in Chinese chives |
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| Institution: | 1.Institute of Biotechnology, Hunan Academy of Agricultural Sciences, Hunan Academy of Agricultural Science, Changsha 410125, China2.Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing 100125, China |
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| Abstract: | An effective method was developed for the determination of procymidone residue in Chinese chives by QuEChERS combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The supervised residue field trials of procymidone were designed and conducted in China (Heilongjiang, Liaoning, Henan, Shandong, Anhui, Hunan, Guangxi, and Fujian Province) in 2019. The risk of residue of procymidone applied in Chinese chives was studied, and the chronic dietary exposure risk of procymidone was evaluated. The samples were extracted by acetonitrile, purified with PSA and GCB, detected using HPLC-MS/MS, and quantified by external standard method. The recoveries ranged from 74% to 98% with the relative standard deviations (RSD) of 4.6%-9.9% at the fortified levels of 0.02 to 20 mg/kg. The limit of quantitation (LOQ) of the method was 0.02 mg/kg. The field trial results showed that after the spraying of 50% wettable powders (WP) of procymidone at 450 g a.i./ hm2 once, the final residues in Chinese chives from 8 provinces were 0.04-1.06 mg/kg at 30 days after the application. Given that the national estimated daily intake (NEDI) of procymidone was 2.5562 mg, its risk quotient (RQ) was 40.6%. The result of the chronic dietary exposure assessment revealed that it might not pose an unacceptable risk to the general population’s health by applying procymidone on Chinese chives. |
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