Adenosine Deaminase Activity in Soils |
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| Authors: | Fumimasa Saio Hiroaki Omura Koichi Hayano |
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| Institution: | Tochigi Agricultural Experiment Station , Utsunomiya , Tochigi , 320 , Japan |
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| Abstract: | Adenosine deaminase activity in six soil samples was measured. One g of moist soil was incubated with 0.02 M adenosine in 0.1 M phosphate buffer (pH 7.6) and toluene in a shaking water bath (30°C) for 72 h. Inosine and NH2 + were detected in the filtrate as degradation products of adenosine; inosine was identified by paper chromatography, and NH2 + was determined by Nessler’s method. The adenosine deaminase activity in soils was completely inhibited by autoclaving (120°C, 15 min). The relation between substrate concentration and reaction rate followed the Michaelis-Menten equation. The optimum pH for adenosine deaminase activity in soils ranged from about 7 to 8. The adenosine deaminase activity in soils ranged from about 1.51 to 4.33 munit (nmol per min) per g of dry soil at 30°C. |
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| Keywords: | adenosine deaminase soils paper chromatography Nessler’s method |
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