| 鸽Ⅰ型副粘病毒F基因的序列分析 |
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| 引用本文： | 王林川,游洪.鸽Ⅰ型副粘病毒F基因的序列分析[J].中国预防兽医学报,2003,25(5):326-329. |
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| 作者姓名： | 王林川 游洪 |
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| 作者单位： | 1. 华南农业大学,兽医学院,广东,广州,510642
2. 广东省出入境检验检疫局,动植物监管处,广东,广州,510623 |
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| 基金项目： | 广东省自然科学基金项目 (NO .980 131)资助 |
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| 摘 要： | 本研究采用RT-PCR方法成功地获得了3个鸽I型副粘病毒广东分离株(P4、P5和P7的F基因片段。经测序表明，基因片段长度均为1290bp；经序列分析发现，毒株P4与NDV标准株La Sota和C30的同源性最高，达到99．4％；而毒P5、P7与La Sota和C30的同源性相对较低，为83％。从建立的系统发育树可看出，毒株P4与NDV标准株La Sota和C30有很近的亲缘关系，而毒株P5、P7与NDV标准株La Sota和C30的亲缘关系相对较远。
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| 关 键 词： | 鸽I型副粘病毒（PPMV-1） RT-PCR F基因 序列分析 |
| 文章编号： | 1008-0589(2003)05-0326-04 |
| 修稿时间： | 2002年12月17 |
Sequence analysis of the F gene of pigeon paramyxovirus type 1 isolates from China |
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| WANG Lin-chuan,YOU Hong.Sequence analysis of the F gene of pigeon paramyxovirus type 1 isolates from China[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(5):326-329. |
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| Authors: | WANG Lin-chuan YOU Hong |
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| Institution: | WANG Lin-chuan~1,YOU Hong~2 |
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| Abstract: | RT-PCR was used to amplify the F gene fragment of three isolates of Pigeon Paramyxovirus type 1 in Guangdong. The result of sequencing assay indicated the F gene fragment of three PPMV-1 isolates consisted of 1 290 bp(not including the sequence of the primers).The sequences of the three PPMV-1 isolates were compared with several NDV standard strains.The result revealed the homology between isolate P4 and NDV standard strains(La Sota and C30)was extremely high,99.4 %,However,there was lower homology for isolates P5 and P7 Compared with La Sota and C30,83 %.The phylogenetic tree indicted isolate P4 was very closely akin to NDV strains La Sota and C30 while isolates P5 and P7 were relatively farther akin to them. |
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| Keywords: | pigeon paramyxovirus type 1 RT-PCR the F gene sequence analysis |
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