| 合肥地区发生的西瓜花叶病的病原鉴定 |
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| 引用本文： | 张大伟,李世访,范卫红,成卓敏,王杰.合肥地区发生的西瓜花叶病的病原鉴定[J].植物病理学报,2004,34(5):474-476. |
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| 作者姓名： | 张大伟 李世访 范卫红 成卓敏 王杰 |
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| 作者单位： | 1 安徽农业大学植物病理教研室, 合肥 230036;2 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100094 |
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| 摘 要： | 从合肥市郊区的西瓜病叶上分离出一病毒分离物,该分离物的热钝化温度为60~65℃,稀释终点为10-4~10-5,寄主体外存活期为20~23 d,电镜观察病毒粒子约750 nm×13 nm。参考已发表的小西葫芦黄化花叶病毒(Zucchini yellow mosaic virus,ZYMV) CP基因序列设计引物,RT-PCR扩增得到CP基因片段。将该CP片段克隆到pGEM-3Zf (+)中,测序结果表明,该CP基因全长840 nt,共编码279个氨基酸,与国内报道的ZYMV CP基因的核苷酸序列同源性为83.0%~97.3%,氨基酸序列的同源性为91.8%~99.8%,证明该分离物为ZYMV。
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| 关 键 词： | 西瓜花叶病 小西葫芦黄化花叶病毒 CP基因 |
| 文章编号： | 0412-0914(2004)05-0474-03 |
| 收稿时间： | 2003-12-09 |
| 修稿时间： | 2003年12月9日 |
Identification of Watermelon mosaic disease in Hefei |
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| ZHANG Da wei ,LI Shi fang ,FAN Wei hong ,CHENG Zhuo min ,WANG Jie.Identification of Watermelon mosaic disease in Hefei[J].Acta Phytopathologica Sinica,2004,34(5):474-476. |
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| Authors: | ZHANG Da wei LI Shi fang FAN Wei hong CHENG Zhuo min WANG Jie |
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| Institution: | 1 Laboratory of Plant Pathology, Anhui Agricultural University, Hefei 230036, China;2 Institute of Plant Protection of Chinese Academy of Agricultural Sciences, The State Key Lab for Biology of Plant Disease and Insect Pest, Beijing 100094, China |
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| Abstract: | A virus isolate, HF-WX, was isolated from infected watermelon in Hefei suburbs. Its dilution end point (DEP) was 10-4-10-5,thermal inactivation point (TIP) was 60-65℃ and longevity in vitro was 20-23 d (20-22℃). The purified virus particles observed under electronic microscope were about 750 nm×13 nm. Synthesizing the primers according to the known Zucchini yellow mosaic virus (ZYMV) coat protein gene sequence, the CP gene was cloned by means of RT-PCR amplification. Then it was inserted into the vector pGEM-3Zf(+). The CP gene was 840 nucleotides determined by sequen cing. Compared with the published sequences inland,it shared identities of 83.0%-97.3% in nucleotide acids and 91.8%-99.8% in amino acids respectively. Based on the above results, it is concluded that this isolate belongs to ZYMV. |
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| Keywords: | Watermelon mosaic disease Zucchini yellow mosaic virus coat protein gene |
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