Identification of the Rf gene conferring fertility restoration of the CMS Dian-type 1 in rice by using simple sequence repeat markers and advanced inbred lines of restorer and maintainer |
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| Authors: | X L Tan Y L Tan Y H Zhao X M Zhang R K Hong S L Jin X R Liu D J Huang |
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| Institution: | Rice Research Institute, Yunnan Agricultural University, Kunming 60201, China, E-mail: rriyau@public.km.yn.cn |
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| Abstract: | Cytoplasmic male sterility of Dian‐type 1 (CMS‐D1) was developed 30 years ago in Yunnan. A major gene conferring fertility restoration for the CMS‐D1 system was detected by microsatellite markers in advanced inbred lines consisting of 196 maintainers and 62 restorers developed in breeding programmes of hybrid rice involving the CMS‐D1 system. The gene was mapped between two simple sequence repeat markers, OSR33 and RM228, on chromosome 10, and was temporarily designated as Rf‐D1(t). The genetic distances of the gene to the two microsatellite markers were 3.4 and 5.0 cM, respectively. This linkage was confirmed by using an F2 population derived from a cross between a CMS‐D1 line and a restorer. This study also demonstrated that using OSR33 was reliable and efficient for identification of restoring lines in hybrid rice breeding with the CMS‐D1 system. |
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| Keywords: | Oryza sativa cytoplasmic male sterility Dian-type 1 fertility-restoring gene japonica rice simple sequence repeat markers |
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