| 不同条件对绒山羊体外培养初级毛囊生长的影响(英文) |
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| 引用本文： | 李蘅,李金泉,曹贵方,张文广.不同条件对绒山羊体外培养初级毛囊生长的影响(英文)[J].农业科学与技术,2008,9(5):43-46. |
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| 作者姓名： | 李蘅 李金泉 曹贵方 张文广 |
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| 作者单位： | 内蒙古农业大学生物工程学院;内蒙古农业大学动物科学与医学学院 |
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| 基金项目： | 国家自然科学基金
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内蒙古自然科学基金
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| 摘 要： | Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium.
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| 关 键 词： | Cashmere goat Primary hair follicle Culture in vitro |
Effects of Different Culture Condition in vitro on Growth of Cashmere Goat Primary Hair Follicles |
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| LI Heng,LI Jin-quan,CAO Gui-fang,ZHANG Wen-guang.Effects of Different Culture Condition in vitro on Growth of Cashmere Goat Primary Hair Follicles[J].Agricultural Science ＆ Technology,2008,9(5):43-46. |
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| Authors: | LI Heng LI Jin-quan CAO Gui-fang ZHANG Wen-guang |
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| Institution: | 1.Bioengineering College,Inner Mongolia Agricultural University,Huhhot 010018;2.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot 010018 |
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| Abstract: | Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles. Method] Cashmere goat primary hair follicles were separated under aseptic condition and cuhured in serum-free DMEM and serum-free Wil-liams E media respectively; subsequently, the growth rate and morphological changes were observed under the inverted microscope. Result] Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days, whose structure and morphological characteristics could maintian a stable status for a long time in the growth process. Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days. Conclusion] There were significant differences(P<0.05) between the growth of cashmere goat hair folhcles cul-tured in the 2 kinds of media. Serum-free Williams E medium was superior to serum-free DMEM medium. |
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| Keywords: | Cashmere goat Primary hair follicle Culture in vitro |
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