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绿僵菌与椰甲截脉姬小蜂对椰心叶甲的协同控制作用
引用本文:边强,王广君,农向群,高松,覃伟权,李洪,张泽华.绿僵菌与椰甲截脉姬小蜂对椰心叶甲的协同控制作用[J].中国生物防治,2010(1):30-34.
作者姓名:边强  王广君  农向群  高松  覃伟权  李洪  张泽华
作者单位:1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室
3. 中国热带农业科学院椰子研究所,文昌571339;
4. 海南省林业局,海口570203;
基金项目:国家科技支撑计划课题(2006BAD08A18)
摘    要:在室内条件下,研究了金龟子绿僵菌小孢变种Metarhizium anisopliae var. anisopliae侵染和椰甲截脉姬小蜂Asecodes hispinarum寄生两种生防措施之间的相互影响,并对两种措施协同控制椰心叶甲Brontispa longissima的效果进行了评价。结果表明,绿僵菌菌株HK4对椰甲截脉姬小蜂成虫安全;但绿僵菌侵染使姬小蜂对椰心叶甲的寄生率显著降低。在先接种绿僵菌后接入姬小蜂的情况下,椰甲截脉姬小蜂对椰心叶甲的寄生率仅为10.23%,显著低于其单独寄生的寄生率79.55%;绿僵菌对椰心叶甲的侵染率为96.59%,高于绿僵菌单独处理的侵染率84.09%,但不显著。而在先接蜂后接种绿僵菌的处理中,绿僵菌对椰心叶甲的侵染率仅为69.09%,显著低于绿僵菌单独处理。对椰心叶甲的生测结果显示,先接种绿僵菌再接入姬小蜂、先姬小蜂寄生再接种绿僵菌、同时接种绿僵菌和接入姬小蜂3种处理的校正死亡率分别为100%、100%、98.86%,显著高于绿僵菌单独侵染和姬小蜂单独寄生。

关 键 词:绿僵菌  椰甲截脉姬小蜂  椰心叶甲  生物防治

Control of Brontispa longissima by Metarhizium anisopliae and Asecodes hispinarum
BIAN Qiang , WANG Guang-jun , NONG Xiang-qun , GAO Song , QIN Wei-quan , LI Hong , ZHANG Ze-hua.Control of Brontispa longissima by Metarhizium anisopliae and Asecodes hispinarum[J].Chinese Journal of Biological Control,2010(1):30-34.
Authors:BIAN Qiang  WANG Guang-jun  NONG Xiang-qun  GAO Song  QIN Wei-quan  LI Hong  ZHANG Ze-hua
Institution:BIAN Qiang1,2,WANG Guang-jun1,NONG Xiang-qun1,GAO Song1,QIN Wei-quan3,LI Hong4,ZHANG Ze-hua1(1.State Key Laboratory for Biology of Plant Diseases , Insect Pests,Institute of Plant Protection,CAAS,Beijing 100193,2.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,3.Coconut Research Institute,CATAS,Wenchang 571339,4.Forestry Bureau of Hainan Province,Haikou 570203,China)
Abstract:Interaction between Metarhizium anisopliae var.anisopliae and Asecodes hispinarum when applied simultaneously to control Brontispa longissima was investigated in the lab,and the efficacy of two strategies above was determined.Results showed that M.anisopliae strain HK4 was safe to the adults of A.hispinarum, while when B.longissima was infected by M.anisopliae at 5×106 conidia/ml ahead,the parasite rate of A.hispinarum decreased sharply from 79.55% to 10.23%.On the other hand,the infection rate of M.anisopliae to B.longissima was also affected by A.hispinarum to some extent,but not significantly.When inoculating firstly with M.anisopliae and then treated with A.hispinarum(96.59%),it was slightly more than that only inoculating with M.anisopliae(84.09%),but when B.longissima was parasitised firstly by A.hispinarum,the infection rate of M.anisopliae was only 69.09%.And the bioassay results showed that when M.anisopliae strain HK4 and A.hispinarum were applied together,its efficacy was about 20% more than that of each strategy used alone.
Keywords:Metarhizium anisopliae var  anisopliae  Asecodes hispinarum  Brontispa longissima  biological control  
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