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| 达里诺尔湖和岗更湖瓦氏雅罗鱼同工酶的研究 | |
| 引用本文： | 张建明,张玉,刘海涛.达里诺尔湖和岗更湖瓦氏雅罗鱼同工酶的研究[J].水生态学杂志,2010,31(6):79-86. |
| 作者姓名： | 张建明 张玉 刘海涛 |
| 作者单位： | 1. 内蒙古农业大学动物科学学院,内蒙古,呼和浩特,010018 2. 内蒙古农业大学动物科学学院,内蒙古,呼和浩特,010018;内蒙古大学生命科学学院,内蒙古,呼和浩特,010021 3. 内蒙古自治区水产技术推广站,内蒙古,呼和浩特,010010 |
| 摘 要： | 瓦氏雅罗鱼是一种耐盐碱性很强的鱼类，本文采用聚丙烯酰胺凝胶电泳法(PAGE)比较研究了淡水水域（岗更湖）和盐碱水水域（达里诺尔湖）中瓦氏雅罗鱼的同工酶，旨在明确瓦氏雅罗鱼在盐碱水域和淡水水域中同工酶的不同，为瓦氏雅罗鱼的耐盐碱机理做基础研究，同时也为瓦氏雅罗鱼在内陆水域的引种与放流提供科学的指导依据。通过比较研究达里诺尔湖和岗根湖的瓦氏雅罗鱼(Leuciscus waleckii)骨骼肌、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)的差异，得出结果：两湖中瓦氏雅罗鱼的同工酶表达具有差异性，其中EST图谱的差异最大，LDH、POD的图谱也存在差异性，而MDH图谱差异不显著。试验表明，两湖中瓦氏雅罗鱼的过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)同工酶存在差异性，而苹果酸脱氢酶(MDH)的差异不显著。 |
| 关 键 词： | 瓦氏雅罗鱼 盐碱水域 同工酶 |
| 收稿时间： | 2009/11/25 0:00:00 |
| 修稿时间： | 2010/3/11 0:00:00 |
Study of Isoenzymes of Leuciscus waleckii in Dalinor-Lake and ganggeng-lake |
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| zhang jianj ming,zhang yu and liu hai tao.Study of Isoenzymes of Leuciscus waleckii in Dalinor-Lake and ganggeng-lake[J].Journal of Hydroecology,2010,31(6):79-86. | |
| Authors: | zhang jianj ming zhang yu and liu hai tao |
| Institution: | Inner Mongolia Agricultural University Animal Science and Medicine Institute,Inner Mongolia Agricultural University Animal Science and Medicine Institute |
| Abstract: | Leuciscus waleckii is a fish that can undertake salinity strongly, in this paper comparatively studied Isoenzymes of Leuciscus waleckii live in salt water and fresh waters with polyacrylamidegel electrophoresis (PAGE), to find difference of Isoenzymes of Leuciscus waleckii live in salt water and fresh water. By studying ,to provide basic research for mechanism of salinity and give scientific guidance for introduced in inland waters and release of Leuciscus waleckii. Through a comparative study peroxidase (POD), esterase (EST), lactate dehydrogenase (LDH)and malic acid de-hydrogenase (MDH) in muscle, liver and heart tissue of Leuciscus waleckii, the results obtained: Leuciscus waleckii with differences in expression of the isozymes in the two lakes, and EST mapping has the biggest differences between them, LDH and POD are also have differences in the pattern, but MDH pattern no significant difference. Experiments show that peroxidase (POD), esterase (EST)and lactate dehydrogenase (LDH)of Leuciscus waleckii have differences in two lakes, whereas the difference of malic acid de - hydrogenase (MDH) was not significant. |
| Keywords: | Leuciscus waleckii Saline waters Isoenzymes |
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