| 辣椒重要农艺性状关联分析与优异等位变异发掘 |
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| 引用本文： | 袁欣捷,方荣,周坤华,雷刚,黄月琴,陈学军.辣椒重要农艺性状关联分析与优异等位变异发掘[J].核农学报,2020,34(12):2658-2672. |
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| 作者姓名： | 袁欣捷 方荣 周坤华 雷刚 黄月琴 陈学军 |
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| 作者单位： | 江西省农业科学院蔬菜花卉研究所,江西 南昌 330200 |
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| 基金项目： | 江西省博士后科研择优资助项目;国家重点研发计划;现代农业产业技术体系建设专项;国家自然科学基金 |
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| 摘 要： | 为了发掘辣椒株高、始花节位、单果质量、果纵径、果横径、果形指数和果肉厚度等重要农艺性状的关联位点和优异等位变异,本研究以194份辣椒核心种质为试验材料,利用广义线性模型(GLM)和混合线性模型(MLM)两种方法,基于分布于辣椒12条染色体的58个SSR标记,对上述7个农艺性状开展了关联分析。结果表明,GLM方法检测到20个SSR关联位点,解释的表型变异率为2.09%~21.91%;MLM方法检测出17个SSR关联位点,能解释2.07%~7.24%的表型变异;有12个位点被两种方法同时检测到;基于关联位点各等位变异的表型效应,发掘出TC7268Sa、CAMS-327a、HpmsE132a、ge35-141pmH0135Ca、CAMS-454c和Hpms2-24a等优异等位变异,筛选出B003、B010、B015、B020、B022、B042、B048、B052、B097、B111、B134、B138、B166、B178、B351、B352、C005、C014、V06C0007、V06C0295、V06C1082、V06C1088、V06C1187、V06C1321、V06C1600、V06C1707、V06C1719和V06C1898等28份优良农艺性状典型种质材料。本研究结果为辣椒相关农艺性状优异基因的发掘、分子标记辅助选择与聚合育种提供了理论指导和材料基础。
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| 关 键 词： | 辣椒 重要农艺性状 关联分析 优异等位变异 |
| 收稿时间： | 2019-11-18 |
Association Analysis of Important Agronomic Traits in Pepper (Capsicum annuum L.) and Mining of Elite Alleles |
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| YUAN Xinjie,FANG Rong,ZHOU Kunhua,LEI Gang,HUANG Yueqin,CHEN Xuejun.Association Analysis of Important Agronomic Traits in Pepper (Capsicum annuum L.) and Mining of Elite Alleles[J].Acta Agriculturae Nucleatae Sinica,2020,34(12):2658-2672. |
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| Authors: | YUAN Xinjie FANG Rong ZHOU Kunhua LEI Gang HUANG Yueqin CHEN Xuejun |
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| Institution: | Vegetable and Flower Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi 330200 |
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| Abstract: | In order to explore the associated loci and elite alleles for important agronomic traits of pepper, including plant height, first flower node, fruit weight, fruit length, fruit diameter, fruit shape index and pericarp thickness, the genotyping data of 58 SSR markers which distributed on 12 chromosomes in 194 Capsicum annuum core collections were used to detect association with 7 phenotypic traits by using GLM (general linear model, GLM) and MLM(mixed linear model, MLM) methods. The results showed that 20 associated SSR loci were detected by GLM methods, which explain 2.09%~21.91% of the phenotypic variance; 17 associated SSR loci were detected by MLM methods, which explain 2.07%~7.24% of the phenotypic variance; 12 loci were detected by both methods; elite alleles such as TC7268Sa, CAMS-327a, HpmsE132a, ge35-141pmH0135Ca, CAMS-454c and Hpms2-24a were explored based on the phenotypic effect of each associated locus; 28 typical excellent carrier materials, including germplasm B003, B010, B015, B020, B022, B042, B048, B052, B097, B111, B134, B138, B166, B178, B351, B352, C005, C014, V06C0007, V06C0295, V06C1082, V06C1088, V06C1187, V06C1321, V06C1600, V06C1707, V06C1719 and V06C1898 were selected based on these elite alleles. The results in this study provided theoretical guidance and valuable natural resources for the excavation of excellent genes, marker-assisted selection breeding and pyramiding breeding for related agronomic traits in pepper. |
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| Keywords: | pepper important agronomic traits association analysis elite alleles |
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