| 气温升高与干旱对灵武长枣果实糖积累、蔗糖代谢关键酶及相关基因表达的影响 |
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| 引用本文： | 陈丽华,杨喜盟,贾昊,宋丽华.气温升高与干旱对灵武长枣果实糖积累、蔗糖代谢关键酶及相关基因表达的影响[J].核农学报,2020,34(9):2112-2123. |
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| 作者姓名： | 陈丽华 杨喜盟 贾昊 宋丽华 |
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| 作者单位： | 宁夏大学农学院,宁夏 银川 750021 |
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| 基金项目： | 国家自然科学基金;创新项目 |
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| 摘 要： | 为探究灵武长枣果实糖积累、蔗糖代谢关键酶及相关基因表达水平对气温升高与干旱的响应,以6年生灵武长枣为试验材料,设置大气环境温度和土壤含水量双因素试验,研究了果实不同成熟期葡萄糖、果糖、蔗糖、淀粉含量及转化酶(AI,NI)、蔗糖合成酶(SS-s,SS-c)、蔗糖磷酸合成酶(SPS)等关键酶活性变化,并从分子水平分析基因表达特性。结果表明,与自然大气温度(T1)环境相比,升高大气温度(T2)环境下灵武长枣果实的葡萄糖、果糖、蔗糖、淀粉含量及NI、SPS、SS-s活性分别增加0.18%~22.02%、0.97%~21.97%、0.43%~85.30%、5.39%~49.06%、0.60%~28.53%、0.01%~31.21%、0.77%~25.31%;与正常土壤供水条件(D1)相比,中度干旱条件(D2)下,灵武长枣果实的果糖、蔗糖、淀粉含量及NI、SS-c活性平均分别增加44.26%、49.56%、38.20%、56.04%、45.36%;大气温度升高2.0℃左右时,ZjNI基因中ZjNI 1和ZjNI 2分别上调14.25和14.52倍;气温升高伴随重度干旱条件下,ZjSPS基因中ZjSPS 1和ZjSPS 4分别上调24.36和93.70倍;ZjSS基因中ZjSS 1和ZjSS 3分别上调131.29倍和81.38倍。综上所述,干旱条件对灵武长枣果实糖含量和关键酶活性的影响显著;气温升高可减缓中度干旱对糖积累的促进作用,加剧重度干旱对糖积累的抑制作用;气温升高伴随干旱可促使蔗糖代谢关键酶基因表达上调。结果表明,大气温度上升和干旱显著影响灵武长枣果实营养品质。本研究可为宁夏灵武长枣生产实践应对全球气候变化提供理论指导与科技支撑。
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| 关 键 词： | 气温升高 干旱 灵武长枣 糖积累 基因表达 |
| 收稿时间： | 2019-08-20 |
Effects of Elevated Temperature and Drought on Sugar Accumulation,Key Sucrose Enzymes Metabolism and Related Gene Expression in Fruit of Jujube Cultivar Lingwuchangzao |
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| CHEN Lihua,YANG Ximeng,JIA Hao,SONG Lihua.Effects of Elevated Temperature and Drought on Sugar Accumulation,Key Sucrose Enzymes Metabolism and Related Gene Expression in Fruit of Jujube Cultivar Lingwuchangzao[J].Acta Agriculturae Nucleatae Sinica,2020,34(9):2112-2123. |
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| Authors: | CHEN Lihua YANG Ximeng JIA Hao SONG Lihua |
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| Institution: | School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021 |
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| Abstract: | Taking 6-year old jujube cultivar Lingwuchangzao (Zizyphus jujube Mill cv.Lingwuchangzao) saplings as experimental subjects, we studied the effect of sugar accumulation, key sucrose enzymes metabolism and related genes expression of Lingwuchangzao on the response of the conditions of elevated air temperature and soil drought. A two-factor experiment of atmospheric temperature and soil water content were set up to study the content of glucose, fructose, sucrose, starch, invertase (AI, NI), sucrose synthase (SS-s, SS-c), sucrose phosphate at different ripening stages synthetase (SPS) and other key enzyme activity changes, and analyze gene expression characteristics from the molecular level. The results showed that compared with the natural air temperature environment (T1), the contents of glucose, fructose, sucrose, starch and the activities of NI, SPS and SS-s increased by 0.18%~22.02%, 0.97%~21.97%, 0.43%~85.30%, 5.39%~49.06%, 0.60%~28.53, 0.01%~31.21% and 0.77%~25.31%, respectively, under the raising the air temperature (T2). Compared with normal soil water supply conditions (D1), the contents of fructose, sucrose, starch, and the activities of NI, SS-c increased by 44.26%, 49.56%, 38.20%, 56.04%, and 45.36%, respectively, under the moderate drought (D2). When the air temperature increased by about 2.0℃, ZjNI 1 and ZjNI 2 in ZjNI gene were up-regulated to 14.25 and 14.52 times, respectively. ZjSPS 1 and ZjSPS 4 in ZjSPS gene were up-regulated by 24.36 and 93.70 times, respectively, and ZjSS 1 and ZjSS 3 in ZjSS gene were up-regulated by 131.29 times and 81.38 times, respectively, under elevated temperature accompanied by severe drought. In conclusion, the effect of drought on sugar content and key enzyme activity in fruit of Lingwuchangzao was significant. The elevated temperature slowed the promotion effect of moderate drought on sugar accumulation, and intensified the inhibiting effect of severe drought on sugar accumulation. The increased temperature and drought promoted the up-regulation of gene expression of key enzyme of sucrose metabolism. This study indicated that the elevated temperature and drought were the most significant factors to the fruit nutrition quality of jujube cultivar Lingwuchangzao, which will provide theoretical guidance and technological support for Lingwuchangzao industry in Ningxia to global climate change. |
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| Keywords: | elevated temperature drought jujube cultivar Lingwuchangzao sugar accumulation gene expression |
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