| 碱茅(Puccinellia tenuiflora)Put-R40g3基因的分离及其与逆境的应答 |
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| 引用本文： | 于雪飞,杨传平.碱茅(Puccinellia tenuiflora)Put-R40g3基因的分离及其与逆境的应答[J].分子植物育种,2009,7(2). |
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| 作者姓名： | 于雪飞 杨传平 |
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| 作者单位： | 1. 东北林业大学林木遗传育种与牛物技术教育部重点实验室,哈尔滨,150040,
2. 东北林业大学林木遗传育种与牛物技术教育部重点实验室,哈尔滨,150040;东北林业大学林木遗传育种黑龙江省重点实验室,哈尔滨,150040 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 从碱茅(Puccinellia tenuiflora)cDNA文库中分离得到Put-R40g3基因(登录号:AB465547).克隆的cDNA全长为588 bp,其编码的蛋白质共195个氨基酸,与水稻,小麦和拟南芥的R40g3蛋白的氨基酸序列有较高的同源性.对经逆境处理的碱茅幼苗根、叶中该基因的表达分析结果表明,碱茅中Put-R40g3基因的表达有被ABA、盐和干旱诱导的趋势.Put-R40g3蛋白与绿色荧光蛋白(GFP)融合在酵母细胞表达的蛋白质定位研究结果显示了Put-R40g3蛋白存在于细胞质中.同时Put-R40g3基因在酵母中表达,酵母在盐、干旱及氧化逆境条件下提高了对逆境的适应能力.这些结果暗示了Put-R40g3基因是一个与逆境相关的基因.
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| 关 键 词： | 碱茅 逆境 酵母 |
Isolation and Characterization of Put-R40g3 Gene in Puccinellia tenuiflora Which Expressed in Response to Abiotic Treatments |
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| Yu Xuefei,Yang Chuanping.Isolation and Characterization of Put-R40g3 Gene in Puccinellia tenuiflora Which Expressed in Response to Abiotic Treatments[J].Molecular Plant Breeding,2009,7(2). |
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| Authors: | Yu Xuefei Yang Chuanping |
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| Institution: | Yu Xuefei 1 Yang Chuanping 1,2 1 Key Laboratory of Forest Tree Genetic Improvement , Biotechnology,Ministry of Education,Northeast Forestry University,Harbin,150040,2 Hei- longjiang Key Laboratory of Forest Tree Genetics , Breeding |
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| Abstract: | Put-R40g3 gene (accession number: AB465547) was isolated from Puccinellia tenuiflora. The ORF of Put-R40g3 gene is 588 bp which encoded 195 amino acids and showed high similarity to R40g3 protein in Triticum aestivum, Oryza sativa and Arabidopsis thaliana. RT-PCR analyses showed that the expression of Put- R40g3 gene in root and leaf of P. tenuiflora was induced by several abiotic stresses from salts, drought and ABA treatment. The Put-R40g3 protein was localized in cytoplasm by using the green fluorescent ... |
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| Keywords: | ABA GFP |
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