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| 反相液相色谱法测定乌桕叶中氨基酸含量 | |
| 引用本文： | 魏京广,霍光华,代建国,詹五根,张平.反相液相色谱法测定乌桕叶中氨基酸含量[J].江西农业学报,2007,19(3):99-101. |
| 作者姓名： | 魏京广 霍光华 代建国 詹五根 张平 |
| 作者单位： | 1. 江西农业大学,生物工程系,江西,南昌,330045;深圳职业技术学院,生物应用工程系,广东,深圳,518055 2. 江西农业大学,生物工程系,江西,南昌,330045 3. 深圳职业技术学院,生物应用工程系,广东,深圳,518055 4. 华南农业大学,动物科学学院,广东,广州,510642 |
| 摘 要： | 以邻苯二甲醛(OPA)与9-芴基羰酰氯(FMOC-CI)作衍生剂,正缬氨酸为内标,利用二极管阵列(DAD)可变波长检测程序,采用柱前衍生RP-HPLC法测定了乌桕叶中氨基酸含量。结果表明:各种氨基酸的保留时间(Rt)、相对峰面积(Ax/AI)的RSD均较满意,回收率为93.21%~114.24%;乌桕叶中含有大量的氨基酸,总得含量在1.3000mg/mL左右,含量较高的是异亮氨酸(Ile)、谷氨酸(G lu)、天冬氨酸(Asp)、亮氨酸(Leu)和精氨酸(Arg),合计含量占总含量的60%以上。18种氨基酸中含有的人体必需氨基酸有7种,其总含量为0.5984mg/mL,占总氨基酸含量的46.03%。 |
| 关 键 词： | 乌桕叶 氨基酸 RP-HPLC 可变波长检测 |
| 文章编号： | 1001-8581（2007）03-0099-03 |
| 收稿时间： | 2006-11-09 |
| 修稿时间： | 2006-11-09 |
Study on Determination of Amino Acids in Chinese Tallow Leaves by RP-HPLC |
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| WEI Jing-guang,HUO Guang-hua,DAI Jian-guo,ZHAN Wu-gen,ZHANG Ping.Study on Determination of Amino Acids in Chinese Tallow Leaves by RP-HPLC[J].Acta Agriculturae Jiangxi,2007,19(3):99-101. | |
| Authors: | WEI Jing-guang HUO Guang-hua DAI Jian-guo ZHAN Wu-gen ZHANG Ping |
| Institution: | 1. Bioengineering Department, Jiangxi Agricultural University, Nanchang 330045 ,China; 2. Department of Applied Biological Engineering, Shenzhen Polytechnic College, Shenzhen 518055 ,China; 3. College of Animal Sciences, South China Agricultural University, Guangzhou 510642,China |
| Abstract: | The amino acids in Chinese tallow leaves were separated and quantified by RP-HPLC after being derived with O-phthaldialdehyde(OPA) and 9-fluorenylmethyl chloroformate(FMOC),and DAD variable-wavelength as measure program.The results showed that the RSD for remaining time and A_x/A_I of amino acids was satisfied,and the recoveries ranged from 93.21% to 114.24%.Chinese tallow leaves contained plenty of amino acids which the total concentration was 1.3000mg/mL.The main amino acids in Chinese tallow leaves were Ile,Glu,Asp,Leu,and Arg,which accounted for more than 60% of total amino acids.The 7 essential amino acids in the 18 amino acids got the amount of 0.5984mg/mL,accounted for 46.03% of the total amino acids. |
| Keywords: | Chinese tallow leaves Amino acids RP-HPLC Variable-wavelength measure |
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