| 10株白僵菌菌株形态学与分子鉴定 |
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| 引用本文： | 刘宝生,谷希树,田涛,霍建飞,孙淑琴.10株白僵菌菌株形态学与分子鉴定[J].山东农业科学,2012,44(6):93-96. |
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| 作者姓名： | 刘宝生 谷希树 田涛 霍建飞 孙淑琴 |
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| 作者单位： | 天津市植物保护研究所,天津,300381 |
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| 基金项目： | 天津市农业科学院院长基金 |
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| 摘 要： | 对10株白僵菌属菌株的菌落形态、产孢结构和分生孢子形状、大小作了观察与测量,形态学鉴定为球孢白僵菌。同时对此10株球孢白僵菌核糖体DNA-ITS进行了测序分析,与NCBI数据库中的球孢白僵菌(Beauveria bassiana)多个菌株的同源性达99.10%～99.64%。依此,10株白僵菌菌株分子鉴定结果为球孢白僵菌(Beauveria bassiana)。
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| 关 键 词： | 球孢白僵菌 菌落形态 分子鉴定 DNA-ITS测序 |
Morphological and Molecular Identification of 10 Beauveria bassiana Strains |
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| LIU Bao-sheng
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GU Xi-shu
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TIAN Tao
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HUO Jian-fei
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SUN Shu-qin.Morphological and Molecular Identification of 10 Beauveria bassiana Strains[J].Shandong Agricultural Sciences,2012,44(6):93-96. |
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| Authors: | LIU Bao-sheng GU Xi-shu TIAN Tao HUO Jian-fei SUN Shu-qin |
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| Institution: | (Tianjin Institute of Plant Protection,Tianjin 300381,China) |
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| Abstract: | In this study,the colony morphology,spore structure and spore shape and size of 10 Beauveria strains were observed and measured,and they were initially identified as Beauveria bassiana.At the same time,their ribosomal DNA-ITS were sequenced and compared with that of Beauveria bassiana in NCBI database.The results showed the homology was 99.10%~99.64%.Eventually these 10 strains were identified as Beauveria bassiana. |
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| Keywords: | Beauveria bassiana Colony morphology Molecular identification DNA-ITS sequence |
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