| 芦笋老茎堆肥中高温纤维素分解菌的分离、筛选及鉴定 |
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| 引用本文： | 李欣欣,王琳琳,申挺挺,宋志刚,韩建荣.芦笋老茎堆肥中高温纤维素分解菌的分离、筛选及鉴定[J].山东农业科学,2012,44(4):81-83. |
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| 作者姓名： | 李欣欣 王琳琳 申挺挺 宋志刚 韩建荣 |
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| 作者单位： | 1. 山西大学生物技术研究所,山西太原,030006
2. 山西大学生命科学学院,山西太原,030006 |
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| 摘 要： | 采用纤维素刚果红培养基在45℃下从芦笋老茎堆肥中分离筛选出52株高温纤维素分解菌。通过水解圈直径与菌落直径比值的比较,进一步获得了6株纤维素分解能力较强的菌株。经菌落形态观察、革兰氏染色、芽孢染色和生理生化试验,6株高温纤维素分解菌初步鉴定为需氧芽孢杆菌(Bacillus spp.)。
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| 关 键 词： | 芦笋老茎 堆肥 高温纤维素分解菌 分离鉴定 |
Isolation, Screening and Identification of Thermophilic Cellulolytic Bacteria in Old Stalk of Asparagus Compost |
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| LI Xin-xin
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WANG Lin-lin
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SHEN Ting-ting
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SONG Zhi-gang
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HAN Jian-rong.Isolation, Screening and Identification of Thermophilic Cellulolytic Bacteria in Old Stalk of Asparagus Compost[J].Shandong Agricultural Sciences,2012,44(4):81-83. |
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| Authors: | LI Xin-xin WANG Lin-lin SHEN Ting-ting SONG Zhi-gang HAN Jian-rong |
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| Institution: | 1.Institute of Biotechnology,Shanxi University,Taiyuan 030006,China; 2.College of Life Science,Shanxi University,Taiyuan 030006,China) |
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| Abstract: | In this experiment,52 strains of thermophilic cellulolytic bacteria were isolated and screened from the old stalk of asparagus compost using cellulose-congo red culture medium at 45℃.By comparing the ratio of hydrolytic circle diameter to colony diameter,6 strains of cellulolytic bacteria with higher efficient were selected,and they were identified as Bacillus spp.through morphological observation,Gram’s staining,spore staining and physiological-biochemical experiments. |
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| Keywords: | Old stalk of asparagus Compost Thermophilic cellulolytic bacteria Isolation and identification |
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