| 大菱鲆胚胎的玻璃化冷冻保存 |
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| 引用本文： | 田永胜.大菱鲆胚胎的玻璃化冷冻保存[J].水产学报,2005,29(2):275-280. |
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| 作者姓名： | 田永胜 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | 国家“十五”863项目(2001AA621100)
农业部海洋渔业资源可持续利用重点开放实验室开放课题(2002-03) |
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| 摘 要： | Vitrification solutions were examined for their suitability of cryopreservation of turbot (Seophthulnms maximus) embryos. PMPI gave higher survival rate (75.68%) than other vitrification solutions and was considered to be suitable liar the cryopreservation of turbot embryos. The freezing point of the vitrification solutions consisted of PG and MeOH in the proportion of 3:2 was measured when the vitrification solutions were cryopreserved. It showed that the vitrification solutions with a cryoprotectant concentration of over 41% have no freezing point and the freezing points of vitrification solutions containing 33.33%-40% cryprotectants were between -32.4℃ and -65.4℃， The freezing point decreased with the increase of cryoprotectant concentration. The resistance of turbot embryos at different stages to the cryoprotectants was studied, It was found that the turbot embryos from 4-5 pairs somite to tail bud were more resistant to cryoprotectants and suitable to vitrify. A live embryo was obtained after cryopreservation in liquid nitrogen for 14h and was hatched out。
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| 关 键 词： | 大菱鲆 胚胎 玻璃化 冷冻保存 |
| 文章编号： | 1000-0615(2005)02-0275-06 |
| 收稿时间： | 2014/3/14 0:00:00 |
| 修稿时间： | 2003年8月5日 |
Cryopreservation of Scophthalmus maximus embryos by vitrification |
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| TIAN Yong-sheng.Cryopreservation of Scophthalmus maximus embryos by vitrification[J].Journal of Fisheries of China,2005,29(2):275-280. |
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| Authors: | TIAN Yong-sheng |
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| Institution: | Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071,China |
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| Abstract: | |
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| Keywords: | Scopluhalmus maximus embryos vitrification cryopreservation |
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