| GA3喷施对川黄柏密植幼林生长及药用成分含量的影响 |
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| 引用本文： | 陈文强,孙文恺,王雅寒,何功秀,何含杰,张党权.GA3喷施对川黄柏密植幼林生长及药用成分含量的影响[J].中南林业科技大学学报,2021(1). |
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| 作者姓名： | 陈文强 孙文恺 王雅寒 何功秀 何含杰 张党权 |
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| 作者单位： | 中南林业科技大学水土保持与荒漠化防治重点实验室;中南林业科技大学生命科学与技术学院;河南农业大学林学院 |
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| 基金项目： | 湖南省重点研发计划项目(2018NK2041);湖南省教育厅重点项目(18A158);长沙市科技局项目(kq1901144);湖南省大学生创新项目(2018105338004)。 |
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| 摘 要： | 【目的】为给川黄柏药用资源早期利用提供理论依据。【方法】以1年生及2年生川黄柏密植幼林个体不同组织为试材,采用不同GA3喷施周期和质量浓度处理,研究各处理在1年生快速生长阶段的生长生理情况,并筛选出最优喷施处理,然后以最优喷施处理,监测分析GA3喷施对2年生川黄柏生长及小檗碱、黄柏碱等药用成分的影响。【结果】1)与CK相比,GA3喷施处理明显促进了1年生川黄柏株高、地径生长以及叶片可溶性糖及可溶性蛋白的积累。其可溶性糖及可溶性蛋白含量与喷施质量浓度呈正相关,与喷施周期呈负相关。最优处理为10 d间隔喷施,质量浓度为100 mg/L的GA3,其株高地径分别为156.76 cm和14.33 mm,较CK分别高了40.44%和20.65%。2)对于2年生密植幼林,与CK相比,GA3喷施处理显著促进枝、叶生长,提高地径、生物量及药用成分含量,且药用成分含量与主干茎的直径大小呈正相关。3)2年生川黄柏各部位药用成分含量高低顺序依次为:主根皮>基部茎皮>侧根皮>中部茎皮>上部茎皮>顶部茎皮,主干110 cm以下部位茎皮药用成分含量完全符合入药规范。【结论】在川黄柏早期栽培中,适当地喷施GA3可明显促进植株的生长和药用成分的积累。在试验喷施周期和喷施质量浓度组合中,表现为短周期、高质量浓度效果明显,且有交互效应,表明了通过经营措施的改进可以提高川黄柏药材品质,增加小檗碱、黄柏碱等药用成分的含量。
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| 关 键 词： | 川黄柏 赤霉素(GA3) 可溶性糖 可溶性蛋白 药用成分 |
Effects of spraying GA on growth and medicinal components content of densely planting young forest of Phellodendron chinense |
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| CHEN Wenqiang,SUN Wenkai,WANG Yahan,HE Gongxiu,HE Hanjie,ZHANG Dangquan.Effects of spraying GA on growth and medicinal components content of densely planting young forest of Phellodendron chinense[J].Journal of Central South Forestry University,2021(1). |
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| Authors: | CHEN Wenqiang SUN Wenkai WANG Yahan HE Gongxiu HE Hanjie ZHANG Dangquan |
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| Institution: | (Key Laboratory of Soil and Water Conservation and Desertification Control,Changsha 410004,Hunan,China;College of Life Sciences and Technology,Central South University of Forestry&Technology,Changsha 410004,Hunan,China;College of Forestry,Henan Agricultural University,Zhengzhou 450002,Henan,China) |
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| Abstract: | 【Objective】In order to provide theoretical basis for the early utilization of medicinal resources of Phellodendron chinense Schneid(PC).【Method】Here different tissues of annual and biennial young plantations of PC were used as experimental materials, different cycles and concentrations of GA3 spraying were used to study the growth and physiology of each treatment group at the rapid growth stage of annual PC, and the optimal spraying treatment was selected. Then the effects of GA3 spraying on the growth and the medicinal components of the biennial PC were monitored and analyzed with the optimal spraying treatment.【Result】1) Compared with CK, GA3 spraying significantly promoted the height and diameter growth, soluble sugar and soluble protein accumulation of PC leaves.The content of soluble sugar and protein was positively correlated with spraying concentration and negatively correlated with spraying cycle before closing.The optimum treatment is 10 days interval and GA3 spraying concentration of 100 mg/L. Its plant height and ground diameter was 156.76 cm and 14.33 mm respectively, which was 40.44% and 20.65% higher than that of the control. 2) For the biennial densely planted young growth, compared with CK, GA3 spraying significantly promoted the growth of branch and leaf, increased the ground diameter, biomass and the content of medicinal ingredients. The content of medicinal ingredients was positively correlated with the diameter of main stem.3) the order of content of medicinal ingredients in different parts of biennial PC is mainroot bark > basal stem bark > lateral root bark > middle stem bark > upper stem bark > apical stem bark. The parts below 110 cm of the main stem bark fully conform to the medication specifications.【Conclusion】In the early cultivation of PC, appropriate application of GA3 can significantly promote plant growth and accumulation of medicinal components. In the combination of spraying period and spraying concentration, the effect of short period and high concentration is obvious, and there is interaction effect.The results showed that the quality of PC could be improved and the contents of berberine could be increasedthrough the adoption of operational measures. |
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| Keywords: | Phellodendron chinense Schneid gibberellic acid3(GA3) soluble sugar soluble protein medicinal ingredients |
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