| 减压耦合超声法提取桑叶脱氧野尻霉素及降血糖活性研究 |
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| 引用本文： | 刘荣,李晓东,毕文玥.减压耦合超声法提取桑叶脱氧野尻霉素及降血糖活性研究[J].中南林业科技大学学报,2021(1). |
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| 作者姓名： | 刘荣 李晓东 毕文玥 |
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| 作者单位： | 东北林业大学林学院;黑龙江省森林食品资源利用重点实验室 |
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| 基金项目： | 国家重点研发计划项目子课题“山野菜保健饮品研发与示范”(2016YFC0500307-07);“十三五”国家重点研发计划项目“东北森林区生态保护及生物资源开发利用技术及示范”(2016YFC0500305)。 |
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| 摘 要： | 【目的】为高效制备桑叶中的一种多羟基生物碱—脱氧野尻霉素(Deoxynojirimycin,DNJ),以期为建立桑叶DNJ提取新技术及其营养价值的开发提供参照。【方法】以桑叶为原料,采用响应面优化法建立基于减压耦合超声法提取桑叶DNJ的工艺条件优化,并以其对α-葡萄糖苷酶与蔗糖酶的综合抑制率为指标,考察其与降血糖活性间的相关性。以阿卡波糖为阳性对照,分别测定了DNJ对α-葡萄糖苷酶、蔗糖酶抑制率的IC50值。【结果】最佳提取工艺条件为料液比1∶40(g∶mL)、超声温度65℃、超声时间24 min、超声功率480 W、乙醇体积分数69%、真空度0.08~0.1 MPa,在此条件下DNJ得率为(1.10±0.02)mg·g-1。与单一超声波法相比提高了64.18%(P <0.01),且桑叶DNJ与降血糖活性之间具有显著相关性r=0.738(P <0.05),以阿卡波糖作为阳性对照,测定桑叶DNJ对α-葡萄糖苷酶和蔗糖酶抑制率的IC50值分别为:0.770、1.772 mg·mL-1。【结论】减压耦合超声波法能高效制备桑叶中的DNJ,使其最终得率明显提升,且桑叶DNJ具有显著的降血糖活性,可作为降血糖功能食品中重要的组成部分。减压耦合超声法使提取容器内空气变稀薄,提取物受到的外力减小,分子链变大,需要打破分子链的能量降低,从而缩短了提取时间,降低了提取温度,减少了能耗。
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| 关 键 词： | 桑叶 脱氧野尻霉素 减压耦合超声法 响应面法 降血糖 |
Effect of ultrasonic-vaccum assisted extraction of deoxynojirimycin from mulberry leaf and its hypoglycemic activity |
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| LIU Rong,LI Xiaodong,BI Wen.Effect of ultrasonic-vaccum assisted extraction of deoxynojirimycin from mulberry leaf and its hypoglycemic activity[J].Journal of Central South Forestry University,2021(1). |
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| Authors: | LIU Rong LI Xiaodong BI Wen |
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| Institution: | (College of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,Heilongjiang,China) |
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| Abstract: | 【Objective】In order to efficiently prepare a deoxynojirimycin(DNJ), a polyhydroxy alkaloid in mulberry leaves, with a view to providing a reference for the development of a new DNJ extraction technology and the nutritional value of mulberry leaves.【Method】Using mulberry leaf as raw material, the response surface optimization method was used to establish the optimization of the process conditions for extracting DNJ from mulberry leaves based on decompression coupled ultrasonic method, and its comprehensive inhibition rate of α-glucosidase and invertase was investigated. Correlation between blood glucose activity. Using acarbose as a positive control, the IC50 values of DNJ inhibition of α-glucosidase and sucrase were measured.【Result】The results showed that the optimum extraction conditions were solid-to-liquid ratio of 1:40(g:mL), ultrasonic temperature of 65℃, ultrasonic time of 24 min, ultrasonic power of 480 W, ethanol volume fraction of 69% and vacuum degree of 0.08~0.1 MPa, under which DNJ was obtained. The rate was(1.10±0.02) mg·g-1. Compared with single ultrasound method, it increased by 64.18%(P < 0.01), and there was a significant correlation between DNJ and hypoglycemic activity of r. serrata r=0.738(P < 0.05). Acarbose was used as positive control to determine mulberry leaves. The IC50 values of DNJ for α-glucosidase and sucrase inhibition rates were 0.770 mg·mL-1 and 1.772 mg·mL-1, respectively.【Conclusion】The decompression coupled ultrasonic method can efficiently prepare DNJ in mulberry leaves, and the final yield is obviously improved. The mulberry leaf DNJ has significant hypoglycemic activity and can be used as an important component in hypoglycemic functional foods. The decompression coupled ultrasonic method makes the air in the extraction container thin, the external force of the extract is reduced, the molecular chain becomes larger, and the energy of the molecular chain needs to be broken, thereby shortening the extraction time, reducing the extraction temperature and reducing the energy consumption. |
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| Keywords: | mulberry leaf deoxynojirimycin ultrasonic-vaccum response surface methodology hypoglycemic |
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