Molecular cloning and expression analysis of genes related to phosphatidic acid synthesis in Japanese pear leaves inoculated with <Emphasis Type="Italic">Venturia nashicola</Emphasis> |
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| Authors: | Qiao-Lin Zheng Hideo Ishii |
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| Institution: | (1) National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba Ibaraki, 305-8604, Japan;(2) Present address: Institute of Life Science and Technology, Hunan University, 410082 Changsha, China |
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| Abstract: | Phosphatidic acid, which can be generated by the action of phospholipase D (PLD) and by the combined action of phospholipase
C (PLC) and diacylglycerol kinase (DGK), is a plant defense signal. To identify the role of the PLD, PLC, and DGK genes encoding these enzymes in pear resistance to the scab fungus Venturia
nashicola, we report the cloning of these genes and analysis of their expression in inoculated pear leaves. The results showed that
PLD mRNA increased in the leaves of the susceptible pear cv. Kousui 1 day after inoculation (dai), then decreased to the basal
level. In resistant pear cv. Kinchaku, PLD mRNA did not change significantly except for a small increase at 2 and 3 dai. However, the level of PLD mRNA in Kinchaku was higher than in Kousui 2 dai. The level of PLC mRNA significantly increased in cvs. Kousui and Kinchaku by 1 dai, peaked at day 2, then decreased to the basal level. Thus,
the gene expression pattern did not differ between the two cultivars. PLC gene expression was also induced by environmental stress. The DGK gene seemed to be constitutively expressed and was not induced by inoculation in either the susceptible or resistant cultivars.
Together, this data showed that both PLD and PLC are possibly involved in a defense response to V. nashicola in Japanese pear leaves, and the PLD gene seems to play a more important role than does the PLC gene. |
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