| 棘托竹荪深层发酵胞外酶活性的研究 |
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| 引用本文： | 李仲芳,李冬琳.棘托竹荪深层发酵胞外酶活性的研究[J].北方园艺,2011(9):197-201. |
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| 作者姓名： | 李仲芳 李冬琳 |
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| 作者单位： | 乐山师范学院,峨眉山生物多样性保护与利用研究所,四川,乐山,614000 |
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| 基金项目： | 乐山市科技重点研究计划资助项目(09N2D007) |
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| 摘 要： | 以棘托竹荪菌株为试材,采用液体培养的方法研究棘托竹荪深层发酵过程中6种胞外酶的酶活性变化,并对菌丝生物量进行了测定。结果表明:酶活性与菌丝生物量增长有密切关系。菌丝生物量增加呈"S"曲线,第4~6天增长最快。棘托竹荪菌丝生物量达到最大值之前,羧甲基纤维素酶和淀粉酶活性出现峰值。过氧化物酶、漆酶、多酚氧化酶和蛋白酶的活性与菌丝生物量同步增加。说明发酵过程中棘托竹荪菌丝首先分解利用淀粉和纤维素,然后利用木质素和蛋白质作为碳源和氮源。要提高棘托竹荪深层发酵效率,缩短培养周期,就必须在其菌丝达到最大生物量之前保证碳源和氮源的均衡供给。可根据不同酶的分泌高峰期,确定菌丝的营养利用情况和发酵周期,以收获最大菌丝生物量。
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| 关 键 词： | 棘托竹荪 菌丝 胞外酶 |
Study on Extracellular Enzyme Activity from Dictyophora echino-volvata Zane during Submerged Fermentation |
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| LI Zhong-fang,LI Dong-lin.Study on Extracellular Enzyme Activity from Dictyophora echino-volvata Zane during Submerged Fermentation[J].Northern Horticulture,2011(9):197-201. |
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| Authors: | LI Zhong-fang LI Dong-lin |
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| Institution: | LI Zhong-fang,LI Dong-lin (Institute of Biodiversity Conservation and Utilization in Mount Emei,Leshan Normal University,Leshan,Sichuan 614000) |
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| Abstract: | Bacterial strain of Dictyophora echino-volvata Zane was used as test material,the activity variation of six extracellular enzymes from Dictyophora echino-volvata Zane during submerged fermentation was studied,by the methods of liquid culture,and the biomass of mycelium were determined.The results showed that the enzyme activity was closely related with the mycelium biomass.Increase in mycelum biomass was of 'S' curve,and the fastest growth of the mycelium occurred at earlier developmental stage,especially t... |
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| Keywords: | Dictyophora echino-volvata Zane mycelium extracellular enzyme |
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