| 酶法脱酯从柚皮中制取低甲氧基果胶的工艺研究 |
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| 引用本文： | 高雪,蔡伟玉,吴礼文,黄伟桓,陈少双,张雪萍.酶法脱酯从柚皮中制取低甲氧基果胶的工艺研究[J].北方园艺,2011(9):191-193. |
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| 作者姓名： | 高雪 蔡伟玉 吴礼文 黄伟桓 陈少双 张雪萍 |
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| 作者单位： | 韩山师范学院生物系,广东,潮州,521041 |
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| 基金项目： | 国家星火计划资助项目(2008GA780034); 韩山师范学院团队科研资助项目(LT200802),韩山师范学院“大学生创新性实验计划”资助项目 |
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| 摘 要： | 以新鲜柚皮为原料,添加碳酸钠激活内源果胶酯酶制备低甲氧基果胶。以果胶甲氧基含量和得率为指标,通过正交实验优化酶法脱酯及酸提取的工艺条件,确定最优低甲氧基果胶制备工艺。结果表明:加入果皮浆液量0.15%的果胶酯酶激活剂碳酸钠,在pH 7.5、45℃的下脱酯50 min,然后采用85℃p、H 3.56、0 min进行酸水解提取果胶。在此工艺条件下制备的果胶甲氧基含量为5.9%,符合低甲氧基果胶标准,且果胶得率较高,为3.0%。
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| 关 键 词： | 低甲氧基果胶 提取 果胶酯酶 柚皮 |
Extraction of Lowmethoxyl Pectin from Grapefuit Peel by Activation Pectin Esterase |
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| GAO Xue,CAI Wei-yu,WU Li-wen,HUANG Wei-huan,CHEN Shao-shuang,ZHANG Xue-ping.Extraction of Lowmethoxyl Pectin from Grapefuit Peel by Activation Pectin Esterase[J].Northern Horticulture,2011(9):191-193. |
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| Authors: | GAO Xue CAI Wei-yu WU Li-wen HUANG Wei-huan CHEN Shao-shuang ZHANG Xue-ping |
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| Institution: | GAO Xue,CAI Wei-yu,WU Li-wen,HUANG Wei-huan,CHEN Shao-shuang,ZHANG Xue-ping(Department of Biology,Hanshan Normal University,Chaozhou,Guangdong 521041) |
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| Abstract: | Lowmethoxyl pectin(LMP)was obtained form fresh grapefruit peel by using Na2CO3 as activator to active pectin esterase in fresh peel.Pectin was de-esterified,then extracted,precipitatied and purified.The results showed that de-esterified temperature,pH level,time,and the percent of Na2CO3 to peel and water were 45℃,pH 7.5,50 min and 0.15%,respectively,and the acid-extraction temperature,pH level and time were 85℃,pH 3.5 and 60 min,respectively.Under this condition,the output rate of pectin was 3.0% with the content of methoxyl below 7%,which was in accordance with the national standard for LMP. |
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| Keywords: | low methoxyl pectin extraction pectin esterase grape fruit peel |
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