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| 银杏苯丙氨酸解氨酶基因的克隆和序列分析 | |
| 引用本文： | 程水源,杜何为,许锋,陈昆松.银杏苯丙氨酸解氨酶基因的克隆和序列分析[J].林业科学研究,2005,18(5):573-577. |
| 作者姓名： | 程水源 杜何为 许锋 陈昆松 |
| 作者单位： | 1. 浙江大学果树研究所,浙江,杭州,310029;长江大学园艺园林学院,湖北,荆州,434025 2. 长江大学生命科学学院,湖北,荆州,434025 3. 浙江大学果树研究所,浙江,杭州,310029 |
| 基金项目： | 湖北省科技厅科技攻关重大项目（2002AB094） |
| 摘 要： | 根据其他植物PAL基因的DNA序列的保守区域，设计了一对简并引物，经PCR扩增，得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序，获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列，与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较，证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录，序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。 |
| 关 键 词： | 银杏 PAL 克隆 Southern blot |
| 文章编号： | 1001-1498（2005）05-0573-05 |
| 收稿时间： | 12 2 2004 12:00AM |
| 修稿时间： | 2004-12-02 |
Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba |
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| CHENG Shui-yuan,DU He-wei,XU Feng and CHEN Kun-song.Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba[J].Forest Research,2005,18(5):573-577. | |
| Authors: | CHENG Shui-yuan DU He-wei XU Feng and CHEN Kun-song |
| Institution: | 1. Fruit Science Institute of Zhejiang University, Hangzhou 310000 ,Zhejiang, China; 2. College of Gardening and Horticulture, Yangtze University, Jingzhou 434025, Hubei ,China; 3. College of Life Science, Yangtze University, Jingzhou 434025, Hubei ,China |
| Abstract: | According to the conservative domain of some other plants DNA sequences of PAL gene, a pair of degenerate primer was designed. A special DNA fragment was obtained by PCR amplifying, which length is 862 bp, and it is named Gbpal1. We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism, the resuits showed that Gbpall is a partial PAL gene of Ginkgo biloba. The sequence had been embodied in Genebank, and Accession NO. is AY578145. The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family. |
| Keywords: | Ginkgo biloba PAL cloning southern blot |
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