| 山羊松果体细胞原代培养及其生物学特性研究 |
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| 引用本文： | 王凌燕,谭雷涛,王树迎.山羊松果体细胞原代培养及其生物学特性研究[J].中国农学通报,2005,21(11):33-33. |
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| 作者姓名： | 王凌燕 谭雷涛 王树迎 |
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| 作者单位： | 山东农业大学动物科技学院 泰安271018 |
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| 基金项目： | 山东省教育厅资助项目（03H03）. |
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| 摘 要： | 目的：观察体外培养山羊松果体细胞的增殖情况和功能状态，为进一步提高季节性繁殖动物的繁殖率提供理论基础。方法：对松果体细胞进行培养，倒置显微镜下观察细胞形态，MTT法检测细胞增殖情况，5-HT免疫组织化学染色显示细胞的功能状态。结果：松果体细胞呈圆形或多角形，折光性强，细胞体积有增大趋势；细胞有明显的增殖现象，大约于11d达到高峰；5-HT阳性细胞数量随细胞的增殖而增加。结论：山羊松果体细胞在体外增殖旺盛。
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| 关 键 词： | 松果体细胞培养 MTT 5-HT免疫组化 山羊 |
| 收稿时间： | 2005-07-13 |
| 修稿时间： | 2005-07-132005-07-18 |
Study on the Biological Characteristics of Primary Cultured Pinealocytes in Goat |
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| Wang Lingyan,Tan Leitao,Wang Shuying.Study on the Biological Characteristics of Primary Cultured Pinealocytes in Goat[J].Chinese Agricultural Science Bulletin,2005,21(11):33-33. |
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| Authors: | Wang Lingyan Tan Leitao Wang Shuying |
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| Institution: | Animal Science CoUe ge,Shandong Agriculture University, Taian 271018 |
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| Abstract: | Objective:To observe and understand the proliferation and functional status of goat pinealocyte in vitro. And produce rational for raising reproduction rate of seasonal breeding animal. Methods:Cells were cultured in routine method. Inverted microscope was used to observe the morphologic feature of pinealocyte. Proliferation and functional status of the cells were measured by MTT and 5-HT immunohis-tochemical method. Results:The pineal cells were round, polygon and glazing.And cells would become much bigger with the time going on.The number of the pinealocytes was greatly increased and reached the peak at the 11th day,which was detected by MTT method.Most pinealocytes contained 5 -HT,including many new born ones.Conclusion:Goat pinealocyte could grow vigorously in vitro culture. |
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| Keywords: | Pinealocyte culture MTT 5-HT immunohistochemistry Goat |
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