| 西瓜花药培养技术研究 |
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| 引用本文： | 李娟,张丽,李焕秀,宫国义,张海英,郭绍贵,许勇.西瓜花药培养技术研究[J].中国瓜菜,2008,21(4). |
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| 作者姓名： | 李娟 张丽 李焕秀 宫国义 张海英 郭绍贵 许勇 |
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| 作者单位： | 1. 四川农业大学林学园艺学院,四川雅安,625014
2. 国家蔬菜工程技术研究中心,北京,100097 |
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| 摘 要： | 以7种不同基因型西瓜为试材进行离体花药培养,对诱导愈伤组织的条件进行了研究。结果表明,西瓜离体花药在MS+KT1.5mg/L+6-BA2.0mg/L+NAA1.0mg/L,MS+KT1.5mg/L+6-BA2.0mg/L+NAA1.5mg/L,MS+KT0.5mg/L+6-BA0.5mg/L+NAA2.0mg/L等3种培养基上愈伤组织诱导率最高,其中野生种331号在MS+KT0.5mg/L+6-BA0.5mg/L+NAA2.0mg/L培养基上诱导率达到了68.42%。将长势较好的愈伤组织转到MS+三十烷醇2.0mg/L+6-BA0.5mg/L培养基上,76%的愈伤组织再生出丛生芽。丛生芽在MS+IBA0.5mg/L的培养基上长出了根,再将其转入1/2MS+IBA0.2mg/L+IAA1.0mg/L培养基上,获得了小植株。
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| 关 键 词： | 西瓜 花药培养 愈伤组织诱导与分化 6-BA 三十烷醇 |
Development of anther culture technique in watermelon(Cirtullus lanatus) |
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| LI Juan,ZHANG Li,LI Huan-xiu,GONG Guo-yi,ZHANG Hai-ying,GUO Sao-gui,XU Yong.Development of anther culture technique in watermelon(Cirtullus lanatus)[J].China Cucurbits And Vegetables,2008,21(4). |
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| Authors: | LI Juan ZHANG Li LI Huan-xiu GONG Guo-yi ZHANG Hai-ying GUO Sao-gui XU Yong |
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| Abstract: | The technology of anther culture was studied by using 7 watermelon varieties. More callus were formed on MS+KT 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.0 mg/L,MS+KT 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.5 mg/L and MS+KT 0.5 mg/L+6-BA 0.5 mg/L+NAA 2.0 mg/L. The wild watermelon 331 produced 68 callus per 100 anthers on MS+0.5 mg/L KT+0.5 mg/L 6-BA +2.0 mg/L NAA. When the selected callus were grown on MS+2.0 mg/L Triacontanol+0.5 mg/L IBA,green shoot formed from the cullus. Plantlets were obtained by transferring the shoots to 1/2 MS+0.2 mg/L IBA+1.0 mg/L IAA. |
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| Keywords: | Watermelon Anther culture Callus induction and differentiation 6-BA Triacontanol |
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